One of the unique characteristics of the Monell Center is that researchers use multidisciplinary approaches to address scientific questions regarding taste, smell, nutrition, and their relationship to human health and well-being. The primary expertise of Monell investigators ranges from analytical chemistry and chemical ecology (Drs. Preti and Kimball) to biochemistry and biophysics (Drs. Brand and Margolskee), molecular and cellular biology (Drs. Margolskee, Huang, and Wang), genetics and neuroscience (Drs. Tordoff, Bachmanov, Beauchamp, Reed. Yamazki, Reisert, Gelperin, and Lowe), endocrinology and physiology (Drs. Margolskee, Teff, Friedman, Wysocki, and Zhao), psychophysics and behavioral science (Drs. Mennella, Dalton, Breslin, Lundstrom, Pelchat, and Wise), and clinical research (Drs. Cowart and Teff). Regardless of their primary technical expertise, a number of these laboratories have found it very useful to incorporate histology and cellular localization methods into their research programs (e.g.. Fig. 1)[1]. However, other Center laboratories are less familiar with the techniques and/or equipment involved in anatomical/histological studies. For instance, geneticists such as Drs. Reed and Bachmanov had identified candidate genes for taste receptors that detect the bitter-tasting compound phenylthiocarbamide and sweet-tasting compounds, respectively[2,3]. They needed to determine, first, whether these genes were expressed in the peripheral taste organs, and then, in which types of taste bud cells. These researchers could use reverse-transcripfion PCR with RNA extracted from taste tissue to determine if these candidate gene products are preferentially expressed in taste buds. However, to determine which type of taste bud cells express these genes, in situ hybridization and immunohistochemistry are mostly commonly used on taste fissures. This Core will provide a common resource and reservoir of experience in chemosensory anatomical analysis and state-of-the-art visualization techniques that would be inefficient to replicate in each individual laboratory. Thus, the availability of Core services would extend the capabilities of the participating laboratories beyond each laboratory's capabilities.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Center Core Grants (P30)
Project #
5P30DC011735-03
Application #
8491770
Study Section
Special Emphasis Panel (ZDC1-SRB-Q)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
3
Fiscal Year
2013
Total Cost
$97,888
Indirect Cost
$24,620
Name
Monell Chemical Senses Center
Department
Type
DUNS #
088812565
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Xu, Mingang; Horrell, Jeremy; Snitow, Melinda et al. (2017) WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation. Nat Commun 8:15397
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Guo, Yiran; Hwang, Liang-Dar; Li, Jiankang et al. (2017) Genetic analysis of impaired trimethylamine metabolism using whole exome sequencing. BMC Med Genet 18:11
Tomassini Barbarossa, Iole; Ozdener, M Hakan; Melis, Melania et al. (2017) Variant in a common odorant-binding protein gene is associated with bitter sensitivity in people. Behav Brain Res 329:200-204
Mennella, Julie A; Mathew, Phoebe S; Lowenthal, Elizabeth D (2017) Use of Adult Sensory Panel to Study Individual Differences in the Palatability of a Pediatric HIV Treatment Drug. Clin Ther 39:2038-2048
Ibarra-Soria, Ximena; Nakahara, Thiago S; Lilue, Jingtao et al. (2017) Variation in olfactory neuron repertoires is genetically controlled and environmentally modulated. Elife 6:
Bobowski, Nuala; Reed, Danielle R; Mennella, Julie A (2016) Variation in the TAS2R31 bitter taste receptor gene relates to liking for the nonnutritive sweetener Acesulfame-K among children and adults. Sci Rep 6:39135

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