The Immunology and Inflammation Core was established in 2010 as a new compo""""""""nent ofthe University of Washington's Diabetes Research Center with the goal of establishing a resource offering state-of-the-art flow cytometry services for the Center's affiliate investigators. To meet this need, the Core's specific aims are to: (1) Provide cost-effective state-of-the-art flow cytometry-related services to characterize and isolate (a) Tand B-lymphocytes and their subpopulations from blood of humans and mice;(b) T- and B-lymphocytes, and myeloid cell populations (macrophages, neutrophils, dendritic cells) from adipose tissue of humans and mice;(c) endothelial cells from several different mouse tissues;and (d) mouse islet endocrine cell populations;(2) Develop new flow cytometry-related techniques to meet affiliate investigators'needs, including development of custom monoclonal antibodies;(3) Provide expert training ih flow cytometry principals and techniques;and (4) Provide consultation in planning, performance, and troubleshooting of flow cytometry analyses. Little more than a year in existence, the core has already provided services to 24 affiliate investigators, which includes the isolation of mouse islet endothelial cells, the'isolation of lymphocytes from mice, and the characterization and isolation of adipose tissue leukcjcyte populations in both humans and mice. Thus, the Immunology and Inflammation Core facilitates and enhances the research of Diabetes Research Center affiliate investigators by providing expertise and state-of-the-art equipment to perform flow cytometry and related techniques that would not otherwise be available to them.

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National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-S (O2))
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Ikizler, Halil O; Zelnick, Leila; Ruzinski, John et al. (2016) Dietary Acid Load is Associated With Serum Bicarbonate but not Insulin Sensitivity in Chronic Kidney Disease. J Ren Nutr 26:93-102
Tang, Chongren; Houston, Barbara A; Storey, Carl et al. (2016) Both STAT3 activation and cholesterol efflux contribute to the anti-inflammatory effect of apoA-I/ABCA1 interaction in macrophages. J Lipid Res 57:848-57
Ronsein, Graziella E; Hutchins, Patrick M; Isquith, Daniel et al. (2016) Niacin Therapy Increases High-Density Lipoprotein Particles and Total Cholesterol Efflux Capacity But Not ABCA1-Specific Cholesterol Efflux in Statin-Treated Subjects. Arterioscler Thromb Vasc Biol 36:404-11
Kanter, Jenny E; Bornfeldt, Karin E (2016) Impact of Diabetes Mellitus. Arterioscler Thromb Vasc Biol 36:1049-53
Shimizu-Albergine, Masami; Van Yserloo, Brian; Golkowski, Martin G et al. (2016) SCAP/SREBP pathway is required for the full steroidogenic response to cyclic AMP. Proc Natl Acad Sci U S A 113:E5685-93
Neal, Adam S; Rountree, Austin M; Radtke, Jared R et al. (2016) A method for high-throughput functional imaging of single cells within heterogeneous cell preparations. Sci Rep 6:39319
Rountree, Austin; Karkamkar, Amit; Khalil, Gamal et al. (2016) BaroFuse, a novel pressure-driven, adjustable-throughput perfusion system for tissue maintenance and assessment. Heliyon 2:e00210
Aroda, Vanita R; Edelstein, Sharon L; Goldberg, Ronald B et al. (2016) Long-term Metformin Use and Vitamin B12 Deficiency in the Diabetes Prevention Program Outcomes Study. J Clin Endocrinol Metab 101:1754-61
Bornfeldt, Karin E (2016) Does Elevated Glucose Promote Atherosclerosis? Pros and Cons. Circ Res 119:190-3
Willard, Joshua R; Barrow, Breanne M; Zraika, Sakeneh (2016) Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels. Diabetologia :

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