The Immunology and Inflammation Core was established in 2010 as a new compo"nent ofthe University of Washington's Diabetes Research Center with the goal of establishing a resource offering state-of-the-art flow cytometry services for the Center's affiliate investigators. To meet this need, the Core's specific aims are to: (1) Provide cost-effective state-of-the-art flow cytometry-related services to characterize and isolate (a) Tand B-lymphocytes and their subpopulations from blood of humans and mice;(b) T- and B-lymphocytes, and myeloid cell populations (macrophages, neutrophils, dendritic cells) from adipose tissue of humans and mice;(c) endothelial cells from several different mouse tissues;and (d) mouse islet endocrine cell populations;(2) Develop new flow cytometry-related techniques to meet affiliate investigators'needs, including development of custom monoclonal antibodies;(3) Provide expert training ih flow cytometry principals and techniques;and (4) Provide consultation in planning, performance, and troubleshooting of flow cytometry analyses. Little more than a year in existence, the core has already provided services to 24 affiliate investigators, which includes the isolation of mouse islet endothelial cells, the'isolation of lymphocytes from mice, and the characterization and isolation of adipose tissue leukcjcyte populations in both humans and mice. Thus, the Immunology and Inflammation Core facilitates and enhances the research of Diabetes Research Center affiliate investigators by providing expertise and state-of-the-art equipment to perform flow cytometry and related techniques that would not otherwise be available to them.
The Immunology and Inflammation Core is a recently established core that uses state-of-the-art equipment and provides the necessary expertise to offer Diabetes Research Center affiliate investigators flow cytometry services.
|Bornfeldt, Karin E (2014) 2013 Russell Ross memorial lecture in vascular biology: cellular and molecular mechanisms of diabetes mellitus-accelerated atherosclerosis. Arterioscler Thromb Vasc Biol 34:705-14|
|Nishizawa, Tomohiro; Kanter, Jenny E; Kramer, Farah et al. (2014) Testing the role of myeloid cell glucose flux in inflammation and atherosclerosis. Cell Rep 7:356-65|
|Baskin, Denis G; Hewitt, Stephen M (2014) Improving the state of the science of immunohistochemistry: the Histochemical Society's standards of practice. J Histochem Cytochem 62:691-2|
|Ho, Jacqueline M; Anekonda, Vishwanath T; Thompson, Benjamin W et al. (2014) Hindbrain oxytocin receptors contribute to the effects of circulating oxytocin on food intake in male rats. Endocrinology 155:2845-57|
|Look AHEAD Research Group (2014) Eight-year weight losses with an intensive lifestyle intervention: the look AHEAD study. Obesity (Silver Spring) 22:5-13|
|Orozco, S; Yatim, N; Werner, M R et al. (2014) RIPK1 both positively and negatively regulates RIPK3 oligomerization and necroptosis. Cell Death Differ 21:1511-21|
|Durinovic-Belló, Ivana; Gersuk, Vivian H; Ni, Chester et al. (2014) Avidity-dependent programming of autoreactive T cells in T1D. PLoS One 9:e98074|
|Martins-Mendes, Daniela; Monteiro-Soares, Matilde; Boyko, Edward John et al. (2014) The independent contribution of diabetic foot ulcer on lower extremity amputation and mortality risk. J Diabetes Complications 28:632-8|
|Kratz, Mario; Marcovina, Santica; Nelson, James E et al. (2014) Dairy fat intake is associated with glucose tolerance, hepatic and systemic insulin sensitivity, and liver fat but not ?-cell function in humans. Am J Clin Nutr 99:1385-96|
|Duffield, Jeremy S (2014) Cellular and molecular mechanisms in kidney fibrosis. J Clin Invest 124:2299-306|
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