The overall objective of the Viral Vector and Transgenic Mouse Core is to provide Diabetes Research Center affiliate investigators at the University of Washington with state-of-the-art vectors necessary to overexpress, knockdown, knockout, or otherwise alter expression of RNAs and proteins of interest in cultured cells, isolated tissues, and animals. The Core has considerably evolved since the last competitive renewal. New services to generate genetically engineered mice have been added, and under-utilized and standard molecular biology methods that are now increasingly performed in individual investigator's labs have been removed.
The specific aims of the Core are to provide the following services to affiliate investigators: (1) Production of lentiviral, adenoviral and retroviral vectors for use in animals, tissues, and cells; (2) Production of vectors for generation of transgenic, knockout, and knockin mice; (3) Specialized molecular biology methods not routinely performed in affiliate investigators' laboratories; (4) Cost-effective production of genetically engineered mice through the University of Washington Transgenic Resources Program; and (5) Consultation and training.. The Core has been highly productive in the current funding period and has added new services that are expected to significantly increase productivity and usability for Diabetes Research Center affiliate investigators to meet the Center's goal to enhance research in diabetes, obesity and related disorders in the Greater Seattle area and beyond.

Public Health Relevance

A cornerstone of diabetes research is the use of viral vectors to modify expression of specific proteins and genetically engineered mice for the study of diabetes pathogenesis, treatment strategies, and diabetic complications. The services provided by the Viral Vector and Transgenic Mouse Core fulfill this need.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK017047-41
Application #
9233082
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2016-12-01
Budget End
2017-11-30
Support Year
41
Fiscal Year
2017
Total Cost
$164,796
Indirect Cost
$68,303
Name
University of Washington
Department
Type
Domestic Higher Education
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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