The long-term goal of the DRC Immunoassay Core is to improve human health by supporting clinical laboratory testing services for research in diabetes mellitus. Since the last competitive review, Washington University School of Medicine has consolidated clinical research testing into the Core Laboratory for Clinical Studies (CLCS), and the DRC Immunoassay Core is fully-integrated into CLCS. This administrative change substantially improves the operational efficiency ofthe DRC Immunoassay Core and provides DRC investigators access to more automated instruments, better-trained personnel and improved quality control procedures. Importantly, institutional support for clinical research testing is focused through CLCS, and the DRC Immunoassay Core leverages this substantial support. CLCS provides expert consultation to investigators so that the most appropriate tests can be chosen while taking cost and number of samples into consideration. DRC investigators are not limited to a fixed menu of tests. Classic metabolic analytes such as insulin and glucagon as well as custom assays such as adiponectin or TNF-a are subsidized. Many other analytes are determined in CLCS at cost. CLCS has a contract with Quest Diagnostics at substantial cost savings for analytes not done at CLCS. Development of new research tests is an important goal. CLCS is currently investigating mouse insulin and plasma VEGF-a (vascular endothelial growth factor alpha) using digital single molecule counting, a promising new technique for materially improving ELISA sensitivity. CLCS is also active in validation of common laboratory analytes for FDA review under industry-sponsored contracts. Developmental research provides equipment and a level of skill that helps investigators both directly and indirectly.
The DRC Immunoassay Core is designed to perform clinical laboratory testing to support Washington University and external research in the field of diabetes mellitus. The primary goals of the Core are to reduce substantially the high costs of specialty testing and to provide outstanding quality control so that reliable diabetes-related tests are widely available to investigators.
|Gaut, Joseph P; Crimmins, Dan L; Ohlendorf, Matt F et al. (2014) Development of an immunoassay for the kidney-specific protein myo-inositol oxygenase, a potential biomarker of acute kidney injury. Clin Chem 60:747-57|
|Hsu, Fong-Fu; Kuhlmann, F Matthew; Turk, John et al. (2014) Multiple-stage linear ion-trap with high resolution mass spectrometry towards complete structural characterization of phosphatidylethanolamines containing cyclopropane fatty acyl chain in Leishmania infantum. J Mass Spectrom 49:201-9|
|Calderon, Boris; Carrero, Javier A; Unanue, Emil R (2014) The central role of antigen presentation in islets of Langerhans in autoimmune diabetes. Curr Opin Immunol 26:32-40|
|Vigueira, Patrick A; McCommis, Kyle S; Schweitzer, George G et al. (2014) Mitochondrial pyruvate carrier 2 hypomorphism in mice leads to defects in glucose-stimulated insulin secretion. Cell Rep 7:2042-53|
|Fabbrini, Elisa; Serafini, Mauro; Colic Baric, Irena et al. (2014) Effect of plasma uric acid on antioxidant capacity, oxidative stress, and insulin sensitivity in obese subjects. Diabetes 63:976-81|
|Asombang, Akwi W; Rahman, Rubayat; Ibdah, Jamal A (2014) Gastric cancer in Africa: current management and outcomes. World J Gastroenterol 20:3875-9|
|Yoshino, Jun; Almeda-Valdes, Paloma; Patterson, Bruce W et al. (2014) Diurnal variation in insulin sensitivity of glucose metabolism is associated with diurnal variations in whole-body and cellular fatty acid metabolism in metabolically normal women. J Clin Endocrinol Metab 99:E1666-70|
|Schugar, Rebecca C; Moll, Ashley R; André d'Avignon, D et al. (2014) Cardiomyocyte-specific deficiency of ketone body metabolism promotes accelerated pathological remodeling. Mol Metab 3:754-69|
|Aguirre, Lina; Napoli, Nicola; Waters, Debra et al. (2014) Increasing adiposity is associated with higher adipokine levels and lower bone mineral density in obese older adults. J Clin Endocrinol Metab 99:3290-7|
|Napoli, Nicola; Shah, Krupa; Waters, Debra L et al. (2014) Effect of weight loss, exercise, or both on cognition and quality of life in obese older adults. Am J Clin Nutr 100:189-98|
Showing the most recent 10 out of 70 publications