The Morphology and Metabolic Analysis Core provides a multidisciplinary approach that integrates structural and functional analysis of diabetes-related tissues. Thus, changes observed in cells or tissues at the histologic and ultrastructural levels, may be correlated to cellular bioenergetic and/or functional changes. The overall objective of the Core is to accelerate the pace, expand the scope, and improve efficiency of diabetes research. This is accomplished through the provision of state-of-the-art technology, services and expertise in the interacting methodologies of histology, electron microscopy, metabolic and functional analysis. The Core services meet the unique requirements of numerous investigators over a wide range of basic and translational research and attract new investigators into diabetes research. These specialized services are not easily replicated within individual laboratories without prohibitive costs in equipment, personnel, and training. In addition, the Core benefits from several institutional departments'financial support of personnel, equipment and space. Importantly the users also benefit from the in-depth diabetes and technical expertise of the Co-directors and staff and the time spent in consultation for experimental design and interpretation of data. In response to a DRC survey, users indicated a significant need to understand the cellular bioenergetics of a wide-range of diabetes-related tissues. To accommodate this evolving need, and to interact with the existing structural services, we have acquired two Seahorse XF Extracellular Flux Analyzers. Other major instrumentation purchased include a transmission EM with a high resolution COD camera and 3D tomography software, two multi-head microscopes for the histology and EM laboratories, a CCD Camera with Metamorph Imaging software for an existing fluorescent microscope, and additional histology equipment. During the current funding period there were 60 users, 53 of these were DRC members, and 45 used multiple services. Users listed 75 grants that were impacted by core services and 3 investigators received pilot and feasibility awards. It is anticipated that requests for core services in histology, EM and functional analysis of diabetes-related tissues will be high in the future considering the recent acquisition of new technologies. Overall, the Morphology and Metabolic Analysis Core has been successful in providing needed services and innovative technology for basic and translational diabetes researchers.
Diabetes affects the cellular structure and metabolic function of numerous tissues. The Morphology and Metabolic Analysis Core provides innovative technology, services and expertise of the Co-directors and technical staff in the interacting methodologies of histology, electron microscopy, metabolic and functional analysis of diabetes-related tissues not available to individual laboratories. The Core provides DRC investigators with increased access to facilities, reduced costs, and collaborative opportunities to enhance the efficiency and productivity of their basic and translational research with the ultimate goal of preventing, treating, and curing diabetes mellitus.
|Gaut, Joseph P; Crimmins, Dan L; Ohlendorf, Matt F et al. (2014) Development of an immunoassay for the kidney-specific protein myo-inositol oxygenase, a potential biomarker of acute kidney injury. Clin Chem 60:747-57|
|Hsu, Fong-Fu; Kuhlmann, F Matthew; Turk, John et al. (2014) Multiple-stage linear ion-trap with high resolution mass spectrometry towards complete structural characterization of phosphatidylethanolamines containing cyclopropane fatty acyl chain in Leishmania infantum. J Mass Spectrom 49:201-9|
|Calderon, Boris; Carrero, Javier A; Unanue, Emil R (2014) The central role of antigen presentation in islets of Langerhans in autoimmune diabetes. Curr Opin Immunol 26:32-40|
|Vigueira, Patrick A; McCommis, Kyle S; Schweitzer, George G et al. (2014) Mitochondrial pyruvate carrier 2 hypomorphism in mice leads to defects in glucose-stimulated insulin secretion. Cell Rep 7:2042-53|
|Fabbrini, Elisa; Serafini, Mauro; Colic Baric, Irena et al. (2014) Effect of plasma uric acid on antioxidant capacity, oxidative stress, and insulin sensitivity in obese subjects. Diabetes 63:976-81|
|Asombang, Akwi W; Rahman, Rubayat; Ibdah, Jamal A (2014) Gastric cancer in Africa: current management and outcomes. World J Gastroenterol 20:3875-9|
|Yoshino, Jun; Almeda-Valdes, Paloma; Patterson, Bruce W et al. (2014) Diurnal variation in insulin sensitivity of glucose metabolism is associated with diurnal variations in whole-body and cellular fatty acid metabolism in metabolically normal women. J Clin Endocrinol Metab 99:E1666-70|
|Schugar, Rebecca C; Moll, Ashley R; André d'Avignon, D et al. (2014) Cardiomyocyte-specific deficiency of ketone body metabolism promotes accelerated pathological remodeling. Mol Metab 3:754-69|
|Aguirre, Lina; Napoli, Nicola; Waters, Debra et al. (2014) Increasing adiposity is associated with higher adipokine levels and lower bone mineral density in obese older adults. J Clin Endocrinol Metab 99:3290-7|
|Napoli, Nicola; Shah, Krupa; Waters, Debra L et al. (2014) Effect of weight loss, exercise, or both on cognition and quality of life in obese older adults. Am J Clin Nutr 100:189-98|
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