The Transgenic and ES Cell Core of the Washington University Diabetes Research Center (DRC) is a shared facility whose goal is the efficient and cost-effective development of genetically-altered mouse models to elucidate the pathogenesis of diabetes and its complications and explore related areas of endocrinology and metabolism. These animal models provide an important avenue for pre-clinical studies that will serve as the basis for translating basic research advances into clinical practice. Importantly, in vivo models allow the analysis of gene function in the context of complex intercellular and integrative systemic responses needed for the study of diabetes, a polygenic disorder involving multiple tissue types and environmental influences. Current molecular genetic techniques allow spatially and temporally restricted analysis of gene effects (both inactivation and over-expression) in mice using altered systems alone or in combination, leading to previously unprecedented creativity and resolution in analysis. Although the concepts involved in generating transgenic (Tg) or knockout/knockin (KO/KI) animals are in general straightforward, the equipment, facilities, and personnel with the appropriate technical skills are either impractical or too expensive for most labs to implement independently. With continued enthusiastic demand for assistance in the generation of genetically altered mice, the DRC Transgenic and ES Cell Core will continue to provide the necessary expertise, facilities, equipment, and personnel to efficiently generate Tg and KO/KI mice for DRC members and assist in propagating pooriy breeding animals. Additional services include marker assisted generation of congenic lines and transient Cre of FLP recombinase expression in zygotes by microinjection of expression plasmids. To accomplish this, the Core will utilize pre-existing, in-house, highly successful facilities - the Washington University Transgenic Vectors Core, Siteman Cancer Center ES Cell Core, and Mouse Genetics Core. Utilization of these facilities for vector design/construction, electroporation, and DNA/ES cell microinjection precludes the need for the Transgenic and ES Cell Core to purchase and set up expensive equipment and independently hire/train/manage our own personnel.

Public Health Relevance

Diabetes is an increasing health problem in the US and around the worid. Generation and analysis of small animal models to studies diabetes and its complications has provided a better understanding of the disease in humans and allowed for the testing of potential therapies. Research facilitated by the services of the DRC Transgenic and ES Cell Core will have a great impact on understanding of human diabetes.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-S (O2))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Washington University
Saint Louis
United States
Zip Code
Burman, Blaire E; Bacchetti, Peter; Khalili, Mandana (2016) Moderate Alcohol Use and Insulin Action in Chronic Hepatitis C Infection. Dig Dis Sci 61:2417-25
Chondronikola, M; Harris, L L S; Klein, S (2016) Bariatric surgery and type 2 diabetes: are there weight loss-independent therapeutic effects of upper gastrointestinal bypass? J Intern Med 280:476-486
Rhee, Julie S; Saben, Jessica L; Mayer, Allyson L et al. (2016) Diet-induced obesity impairs endometrial stromal cell decidualization: a potential role for impaired autophagy. Hum Reprod 31:1315-26
Siller, Alejandro F; Lugar, Heather; Rutlin, Jerrel et al. (2016) Severity of clinical presentation in youth with type 1 diabetes is associated with differences in brain structure. Pediatr Diabetes :
Lin, Jonathan B; Kubota, Shunsuke; Ban, Norimitsu et al. (2016) NAMPT-Mediated NAD(+) Biosynthesis Is Essential for Vision In Mice. Cell Rep 17:69-85
Jarad, George; Knutsen, Russell H; Mecham, Robert P et al. (2016) Albumin contributes to kidney disease progression in Alport syndrome. Am J Physiol Renal Physiol 311:F120-30
Westbroek, Wendy; Nguyen, Matthew; Siebert, Marina et al. (2016) A new glucocerebrosidase-deficient neuronal cell model provides a tool to probe pathophysiology and therapeutics for Gaucher disease. Dis Model Mech 9:769-78
Merriwether, Ericka N; Hastings, Mary K; Bohnert, Kathryn L et al. (2016) Impact of foot progression angle modification on plantar loading in individuals with diabetes mellitus and peripheral neuropathy. Edorium J Disabil Rehabil 2:15-23
Chowdhury, Sara; Wang, Songyan; Dunai, Judit et al. (2016) Hormonal Responses to Cholinergic Input Are Different in Humans with and without Type 2 Diabetes Mellitus. PLoS One 11:e0156852
Zou, Wei; Rohatgi, Nidhi; Chen, Timothy Hung-Po et al. (2016) PPAR-γ regulates pharmacological but not physiological or pathological osteoclast formation. Nat Med 22:1203-1205

Showing the most recent 10 out of 487 publications