The Transgenic and ES Cell Core of the Washington University Diabetes Research Center (DRC) is a shared facility whose goal is the efficient and cost-effective development of genetically-altered mouse models to elucidate the pathogenesis of diabetes and its complications and explore related areas of endocrinology and metabolism. These animal models provide an important avenue for pre-clinical studies that will serve as the basis for translating basic research advances into clinical practice. Importantly, in vivo models allow the analysis of gene function in the context of complex intercellular and integrative systemic responses needed for the study of diabetes, a polygenic disorder involving multiple tissue types and environmental influences. Current molecular genetic techniques allow spatially and temporally restricted analysis of gene effects (both inactivation and over-expression) in mice using altered systems alone or in combination, leading to previously unprecedented creativity and resolution in analysis. Although the concepts involved in generating transgenic (Tg) or knockout/knockin (KO/KI) animals are in general straightforward, the equipment, facilities, and personnel with the appropriate technical skills are either impractical or too expensive for most labs to implement independently. With continued enthusiastic demand for assistance in the generation of genetically altered mice, the DRC Transgenic and ES Cell Core will continue to provide the necessary expertise, facilities, equipment, and personnel to efficiently generate Tg and KO/KI mice for DRC members and assist in propagating pooriy breeding animals. Additional services include marker assisted generation of congenic lines and transient Cre of FLP recombinase expression in zygotes by microinjection of expression plasmids. To accomplish this, the Core will utilize pre-existing, in-house, highly successful facilities - the Washington University Transgenic Vectors Core, Siteman Cancer Center ES Cell Core, and Mouse Genetics Core. Utilization of these facilities for vector design/construction, electroporation, and DNA/ES cell microinjection precludes the need for the Transgenic and ES Cell Core to purchase and set up expensive equipment and independently hire/train/manage our own personnel.
Diabetes is an increasing health problem in the US and around the worid. Generation and analysis of small animal models to studies diabetes and its complications has provided a better understanding of the disease in humans and allowed for the testing of potential therapies. Research facilitated by the services of the DRC Transgenic and ES Cell Core will have a great impact on understanding of human diabetes.
|Rusconi, B; Jiang, X; Sidhu, R et al. (2018) Gut Sphingolipid Composition as a Prelude to Necrotizing Enterocolitis. Sci Rep 8:10984|
|Chen, Yana; McCommis, Kyle S; Ferguson, Daniel et al. (2018) Inhibition of the Mitochondrial Pyruvate Carrier by Tolylfluanid. Endocrinology 159:609-621|
|Zhang, Yan; Rohatgi, Nidhi; Veis, Deborah J et al. (2018) PGC1? Organizes the Osteoclast Cytoskeleton by Mitochondrial Biogenesis and Activation. J Bone Miner Res 33:1114-1125|
|Xu, Wei; Mukherjee, Sumit; Ning, Yu et al. (2018) Cyclopropane fatty acid synthesis affects cell shape and acid resistance in Leishmania mexicana. Int J Parasitol 48:245-256|
|Hughes, Jing W; Bao, Yicheng K; Salam, Maamoun et al. (2018) Late-Onset T1DM and Older Age Predict Risk of Additional Autoimmune Disease. Diabetes Care :|
|Zhang, Xiangyu; Evans, Trent D; Jeong, Se-Jin et al. (2018) Classical and alternative roles for autophagy in lipid metabolism. Curr Opin Lipidol 29:203-211|
|Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration. J Lipid Res 59:1414-1423|
|Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Impaired monocyte cholesterol clearance initiates age-related retinal degeneration and vision loss. JCI Insight 3:|
|Mayer, Allyson L; Zhang, Yiming; Feng, Emily H et al. (2018) Enhanced Hepatic PPAR? Activity Links GLUT8 Deficiency to Augmented Peripheral Fasting Responses in Male Mice. Endocrinology 159:2110-2126|
|Weber, Kassandra J; Sauer, Madeline; He, Li et al. (2018) PPAR? Deficiency Suppresses the Release of IL-1? and IL-1? in Macrophages via a Type 1 IFN-Dependent Mechanism. J Immunol 201:2054-2069|
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