The Mass Spectrometry (MS) Core of the Washington University Diabetes Research Center (DRC) increases DRC efficiency and cost effectiveness by performing MS analyses for affiliated investigators. The MS Core provides state-of-the-art MS services to determine structures and to quantitate amounts of diabetes-related biomolecules. The MS Core provides centralized, standardized analytical procedures that permit study of molecular mechanisms of the pathogenesis of diabetes mellitus, its risk factors, and its complications, including the metabolic syndrome, obesity, atherosclerosis, and increased susceptibility to infections. Speciflc objectives of the DRC MS Core are: 1. To provide training to students and fellows in principles of MS and use of MS systems, e.g., gas chromatography (GC)/MS, isotope ratio (IR)/MS, electrospray ionization (ESI)/tandem MS, and matrix assisted laser desorption ionization (MALDI)/time of fiight/(TOF)/MS in analyses of diabetes-related biomolecules. To develop new MS methods for structural identification and quantitation of molecules involved in diabetes, its risk factors and complications, and related physiologic and pathophysiologic events. To perform service MS analyses, e.g. quantitation of target analytes and obtaining spectra for structural i identification, for DRTC Investigators. To assist DRC-affiliated investigators in developing MS assays. To provide consultation in interpreting MS data. To develop and disseminate new approaches in biomedical MS that are applicable to diabetes research. To provide and maintain functional MS systems for use in diabetes research. To perform collaborative diabetes research studies involving MS Core staff requiring specialized expertise, e.g.. Stable Isotope Labeling in Cell Culture (SILAC), Stable Isotpe Labeling Tandem Mass Spectrometry (SILT), characterization of post-translational modifications or protein-protein interactions, or studies of complex lipids that require de novo structure determination. 9. To reduce diabetes research costs by providing centralized MS services at a fraction of the cost of commercial MS services or of maintaining instruments in the laboratories of individual investigators.

Public Health Relevance

The DRC MS Core employs the power of mass spectrometry to study biochemical and metabolic pathways that are altered in diabetes and in its risk factors and complications, including obesity, cardiovascular disease, and increased suscpetibility to infections, which cause morbidity and accelerate mortality. Insight into their mechanisms may lead to more effective means to prevent or treat them.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-S)
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Washington University
Saint Louis
United States
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Burman, Blaire E; Bacchetti, Peter; Khalili, Mandana (2016) Moderate Alcohol Use and Insulin Action in Chronic Hepatitis C Infection. Dig Dis Sci 61:2417-25
Chondronikola, M; Harris, L L S; Klein, S (2016) Bariatric surgery and type 2 diabetes: are there weight loss-independent therapeutic effects of upper gastrointestinal bypass? J Intern Med 280:476-486
Rhee, Julie S; Saben, Jessica L; Mayer, Allyson L et al. (2016) Diet-induced obesity impairs endometrial stromal cell decidualization: a potential role for impaired autophagy. Hum Reprod 31:1315-26
Siller, Alejandro F; Lugar, Heather; Rutlin, Jerrel et al. (2016) Severity of clinical presentation in youth with type 1 diabetes is associated with differences in brain structure. Pediatr Diabetes :
Lin, Jonathan B; Kubota, Shunsuke; Ban, Norimitsu et al. (2016) NAMPT-Mediated NAD(+) Biosynthesis Is Essential for Vision In Mice. Cell Rep 17:69-85
Jarad, George; Knutsen, Russell H; Mecham, Robert P et al. (2016) Albumin contributes to kidney disease progression in Alport syndrome. Am J Physiol Renal Physiol 311:F120-30
Westbroek, Wendy; Nguyen, Matthew; Siebert, Marina et al. (2016) A new glucocerebrosidase-deficient neuronal cell model provides a tool to probe pathophysiology and therapeutics for Gaucher disease. Dis Model Mech 9:769-78
Merriwether, Ericka N; Hastings, Mary K; Bohnert, Kathryn L et al. (2016) Impact of foot progression angle modification on plantar loading in individuals with diabetes mellitus and peripheral neuropathy. Edorium J Disabil Rehabil 2:15-23
Chowdhury, Sara; Wang, Songyan; Dunai, Judit et al. (2016) Hormonal Responses to Cholinergic Input Are Different in Humans with and without Type 2 Diabetes Mellitus. PLoS One 11:e0156852
Zou, Wei; Rohatgi, Nidhi; Chen, Timothy Hung-Po et al. (2016) PPAR-γ regulates pharmacological but not physiological or pathological osteoclast formation. Nat Med 22:1203-1205

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