The Islet Cell Biology Core provides services and hands-on training to independently funded investigators in the isolation and functional characterization of pancreatic islets from normal and diabetic humans and mice. It also maintains a repository of insulinoma cell lines as well as other endocrine cell lines. The primary emphasis of the Core is to facilitate studies of primary islet cells, and it has developed many unique tools and techniques for carrying such studies including novel animals models, biophysical methods and a library of adenovirus-based expression constructs for studying beta-cell function. The long-range objectives and goals of the Islet Cell Biology Core are to provide state-of-the-art technology and know-how to understanding the beta cell in health and disease. It has the following Specific Aims and Objectives: Provide advice, service and training in the isolation of pancreatic islets from normal and diabetic mice and humans;Provide insulinoma and other endocrine cell lines;Provide advice, service and training in the characterization of mouse and human pancreatic islets and beta cell (insulin biosynthesis and secretion;biophysical methods for studying islet and beta-cell function (e.g. calcium imaging, electrophysiology, total internal reflection fluorescent microscopy);biochemical methods for studying beta-cell function (e.g. phosphorylation, proliferation, apoptosis);and immunohistochemical analysis of pancreatic islets) and Provide advice, service and training on the use of adenovirus-based expression constructs to study protein function in beta cells and other cell types in vitro and in vivo. Drs. Philipson, Rhodes and Witkowski are Directors of this Core. They will be advised by experts in pancreatic islets and beta cells (Prince and Steiner), cellular and physiological imaging (Bindokas, Chen and Glick), ion channels (Hanck and Nelson), spectroscopy (Halpern and Scherer) and cell dynamics (Dinner) to ensure that the Core services are at the forefront of technology and thus able to anticipate and quickly repond to users needs.

Public Health Relevance

The Islet Cell Biology Core provides advice, services and hands-on training in the isolation of pancreatic islets and studies of their function. It provides a foundation for studies of beta cell and islet biology that are fundamental to understanding the pathophysiology of diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK020595-36
Application #
8446544
Study Section
Special Emphasis Panel (ZDK1-GRB-S (O2))
Project Start
2013-02-01
Project End
2018-01-31
Budget Start
2013-02-26
Budget End
2014-01-31
Support Year
36
Fiscal Year
2013
Total Cost
$228,615
Indirect Cost
$65,568
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Bergeron, Valérie; Ghislain, Julien; Vivot, Kevin et al. (2018) Deletion of Protein Kinase D1 in Pancreatic ?-Cells Impairs Insulin Secretion in High-Fat Diet-Fed Mice. Diabetes 67:71-77
Chen, Han; Cade, Brian E; Gleason, Kevin J et al. (2018) Multiethnic Meta-Analysis Identifies RAI1 as a Possible Obstructive Sleep Apnea-related Quantitative Trait Locus in Men. Am J Respir Cell Mol Biol 58:391-401
Go??b, Karolina; Grose, Randall; Placencia, Veronica et al. (2018) Cell banking for regulatory T cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotype. Oncotarget 9:9728-9740
Xiang, Anny H; Trigo, Enrique; Martinez, Mayra et al. (2018) Impact of Gastric Banding Versus Metformin on ?-Cell Function in Adults With Impaired Glucose Tolerance or Mild Type 2 Diabetes. Diabetes Care 41:2544-2551
Reardon, Catherine A; Lingaraju, Amulya; Schoenfelt, Kelly Q et al. (2018) Obesity and Insulin Resistance Promote Atherosclerosis through an IFN?-Regulated Macrophage Protein Network. Cell Rep 23:3021-3030
Priyadarshini, Medha; Kotlo, Kumar U; Dudeja, Pradeep K et al. (2018) Role of Short Chain Fatty Acid Receptors in Intestinal Physiology and Pathophysiology. Compr Physiol 8:1091-1115
Kirkley, Andrew G; Carmean, Christopher M; Ruiz, Daniel et al. (2018) Arsenic exposure induces glucose intolerance and alters global energy metabolism. Am J Physiol Regul Integr Comp Physiol 314:R294-R303
Spires, Denisha; Ilatovskaya, Daria V; Levchenko, Vladislav et al. (2018) Protective role of Trpc6 knockout in the progression of diabetic kidney disease. Am J Physiol Renal Physiol 315:F1091-F1097
Vaicik, Marcella K; Blagajcevic, Alen; Ye, Honggang et al. (2018) The Absence of Laminin ?4 in Male Mice Results in Enhanced Energy Expenditure and Increased Beige Subcutaneous Adipose Tissue. Endocrinology 159:356-367
Yamamoto, Soh; Kuramoto, Kenta; Wang, Nan et al. (2018) Autophagy Differentially Regulates Insulin Production and Insulin Sensitivity. Cell Rep 23:3286-3299

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