The Islet Cell Biology Core provides services and hands-on training to independently funded investigators in the isolation and functional characterization of pancreatic islets from normal and diabetic humans and mice. It also maintains a repository of insulinoma cell lines as well as other endocrine cell lines. The primary emphasis of the Core is to facilitate studies of primary islet cells, and it has developed many unique tools and techniques for carrying such studies including novel animals models, biophysical methods and a library of adenovirus-based expression constructs for studying beta-cell function. The long-range objectives and goals of the Islet Cell Biology Core are to provide state-of-the-art technology and know-how to understanding the beta cell in health and disease. It has the following Specific Aims and Objectives: Provide advice, service and training in the isolation of pancreatic islets from normal and diabetic mice and humans;Provide insulinoma and other endocrine cell lines;Provide advice, service and training in the characterization of mouse and human pancreatic islets and beta cell (insulin biosynthesis and secretion;biophysical methods for studying islet and beta-cell function (e.g. calcium imaging, electrophysiology, total internal reflection fluorescent microscopy);biochemical methods for studying beta-cell function (e.g. phosphorylation, proliferation, apoptosis);and immunohistochemical analysis of pancreatic islets) and Provide advice, service and training on the use of adenovirus-based expression constructs to study protein function in beta cells and other cell types in vitro and in vivo. Drs. Philipson, Rhodes and Witkowski are Directors of this Core. They will be advised by experts in pancreatic islets and beta cells (Prince and Steiner), cellular and physiological imaging (Bindokas, Chen and Glick), ion channels (Hanck and Nelson), spectroscopy (Halpern and Scherer) and cell dynamics (Dinner) to ensure that the Core services are at the forefront of technology and thus able to anticipate and quickly repond to users needs.

Public Health Relevance

The Islet Cell Biology Core provides advice, services and hands-on training in the isolation of pancreatic islets and studies of their function. It provides a foundation for studies of beta cell and islet biology that are fundamental to understanding the pathophysiology of diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK020595-36
Application #
8446544
Study Section
Special Emphasis Panel (ZDK1-GRB-S (O2))
Project Start
2013-02-01
Project End
2018-01-31
Budget Start
2013-02-26
Budget End
2014-01-31
Support Year
36
Fiscal Year
2013
Total Cost
$228,615
Indirect Cost
$65,568
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Smith, Erika; Greeley, Siri Atma W; Ye, Honggang et al. (2016) Extremely Early Onset IPEX Syndrome Caused by a Novel Small Exonic Deletion in FOXP3. J Pediatr Gastroenterol Nutr 63:e119-e120
(2016) Trans-ancestry meta-analyses identify rare and common variants associated with blood pressure and hypertension. Nat Genet 48:1151-61
Alarcon, Cristina; Boland, Brandon B; Uchizono, Yuji et al. (2016) Pancreatic β-Cell Adaptive Plasticity in Obesity Increases Insulin Production but Adversely Affects Secretory Function. Diabetes 65:438-50
Sathe, Neha A; Nocon, Robert S; Hughes, Brenna et al. (2016) The Costs of Participating in a Diabetes Quality Improvement Collaborative: Variation Among Five Clinics. Jt Comm J Qual Patient Saf 42:18-25
(2016) The genetic architecture of type 2 diabetes. Nature 536:41-7
Yi, Yaling; Sun, Xingshen; Gibson-Corley, Katherine et al. (2016) A Transient Metabolic Recovery from Early Life Glucose Intolerance in Cystic Fibrosis Ferrets Occurs During Pancreatic Remodeling. Endocrinology 157:1852-65
Hoang, Danh-Tai; Hara, Manami; Jo, Junghyo (2016) Design Principles of Pancreatic Islets: Glucose-Dependent Coordination of Hormone Pulses. PLoS One 11:e0152446
Sun, Juan; Mao, Li-Qun; Polonsky, Kenneth S et al. (2016) Pancreatic β-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak. J Biol Chem 291:13529-34
Volden, Paul A; Skor, Maxwell N; Johnson, Marianna B et al. (2016) Mammary Adipose Tissue-Derived Lysophospholipids Promote Estrogen Receptor-Negative Mammary Epithelial Cell Proliferation. Cancer Prev Res (Phila) 9:367-78
Horikoshi, Momoko; Pasquali, Lorenzo; Wiltshire, Steven et al. (2016) Transancestral fine-mapping of four type 2 diabetes susceptibility loci highlights potential causal regulatory mechanisms. Hum Mol Genet 25:2070-2081

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