The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune function in mucosal surfaces and solid organs of the GI tract. Specific activities and services include: Polarized Epithelial Cell Culture and Genetic Transformations nCounter NanoString RNA Analysis FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells Transepithelial Electrophysiology of live cells, cell monolayers, and tissues Gastrointestinal Organoid Derivation and Culture Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and purification, including small molecules, specialized and unique epithelial cell lines, expression profiling using Bio-Plex MAGPIX multiplex reader Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and FRAP analysis of membrane molecules A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded >4000 billable hours/year. 45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be utilized.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK034854-31
Application #
8971192
Study Section
Special Emphasis Panel ()
Project Start
Project End
Budget Start
2015-12-01
Budget End
2016-11-30
Support Year
31
Fiscal Year
2016
Total Cost
$252,287
Indirect Cost
$93,753
Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115
Hong, Shangyu; Song, Wei; Zushin, Peter-James H et al. (2018) Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Mol Metab 12:25-38
Aden, Konrad; Tran, Florian; Ito, Go et al. (2018) ATG16L1 orchestrates interleukin-22 signaling in the intestinal epithelium via cGAS-STING. J Exp Med 215:2868-2886
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O'Connell, Amy E; Zhou, Fanny; Shah, Manasvi S et al. (2018) Neonatal-Onset Chronic Diarrhea Caused by Homozygous Nonsense WNT2B Mutations. Am J Hum Genet 103:131-137
Allegretti, J R; Allegretti, A S; Phelps, E et al. (2018) Asymptomatic Clostridium difficile carriage rate post-fecal microbiota transplant is low: a prospective clinical and stool assessment. Clin Microbiol Infect 24:780.e1-780.e3

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