Tissue morphology and knowledge of the localization of specific proteins within a cell are integral to understanding cellular metabolism and the causes and consequences of diabetes and new advances for the treatment of diabetes. The tools of morphological analysis are varied but most involve particular skills or equipment that are too costly to be supported in individual laboratories. The objective of the Advanced Microscopy Core has been to make sophisficated morphological techniques readily available to all invesfigators ofthe Joslin DRC. The Core provides a wide range of services covering the broad categories: performing specific morphological procedures, providing access to equipment and reagents, and giving advice, training and interpretation. There are five main components in this core: Electron Microscopy (EM), Confocal, Histology and Laser Capture Dissecfion (LCM) facilifies and consultafion. Each of the components have specific operafional modes: the plastic embedding and electron microscopic work is done by the Core Manager, a senior highly experienced electron microscopist;the Core Histology Technicians take fixed/frozen fissue and returns both H&E and unstained slides for examinafion by the investigator. Both the Confocal microscopes and Laser Capture Dissecfion equipment are used direcfiy by the individual investigators with the Core providing training of new users, maintenance of the microscopes, and troubleshooting problems. The ultimate goal in all core activities is to provide prompt, customized, high-quality service. The core personnel are readily available for providing advice, troubleshooting and assistance with data interpretation to the users. The core provides every reasonable effort to help DRC investigators have access to a wide range of cutfing-edge morphological techniques.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Joslin Diabetes Center
United States
Zip Code
Mezza, Teresa; Sorice, Gian P; Conte, Caterina et al. (2016) β-Cell Glucose Sensitivity Is Linked to Insulin/Glucagon Bihormonal Cells in Nondiabetic Humans. J Clin Endocrinol Metab 101:470-5
Shirakawa, J; Kulkarni, R N (2016) Novel factors modulating human β-cell proliferation. Diabetes Obes Metab 18 Suppl 1:71-7
Bonner-Weir, Susan; Aguayo-Mazzucato, Cristina (2016) Physiology: Pancreatic β-cell heterogeneity revisited. Nature 535:365-6
Hettmer, Simone; Lin, Michael M; Tchessalova, Daria et al. (2016) Hedgehog-driven myogenic tumors recapitulate skeletal muscle cellular heterogeneity. Exp Cell Res 340:43-52
Kokoye, Yasin; Ivanov, Ivan; Cheng, Qiufang et al. (2016) A comparison of the effects of factor XII deficiency and prekallikrein deficiency on thrombus formation. Thromb Res 140:118-24
Vegas, Arturo J; Veiseh, Omid; Doloff, Joshua C et al. (2016) Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primates. Nat Biotechnol 34:345-52
Khamaisi, Mogher; Katagiri, Sayaka; Keenan, Hillary et al. (2016) PKCδ inhibition normalizes the wound-healing capacity of diabetic human fibroblasts. J Clin Invest 126:837-53
Garvey, Katharine C; Telo, Gabriela H; Needleman, Joseph S et al. (2016) Health Care Transition in Young Adults With Type 1 Diabetes: Perspectives of Adult Endocrinologists in the U.S. Diabetes Care 39:190-7
Bonner-Weir, Susan; Aguayo-Mazzucato, Cristina; Weir, Gordon C (2016) Dynamic development of the pancreas from birth to adulthood. Ups J Med Sci 121:155-8
Ogawa, Takahiro; Kodera, Yukihiro; Hirata, Dai et al. (2016) Natural thioallyl compounds increase oxidative stress resistance and lifespan in Caenorhabditis elegans by modulating SKN-1/Nrf. Sci Rep 6:21611

Showing the most recent 10 out of 933 publications