PURPOSE: The Cell Culture and Genetic Engineering Core has two components: I. Cell culture component: Objectives of this component are to provide Liver Research Center Investigators with: (i) isolated liver cells of consistently high quality, (iii) well-characterized frozen stocks of cell lines, (iv) specialized culture media, hormones and supplements, and matrix components for optimal cell growth; and (iv) equipment and instructions for use in isolation and culturing of various cell types derived from the liver and II. Genetic Engineering component: (i) The objectives are to assist Liver Center Investigators in developing recombinant viral vectors, (based on oncoretroviruses, lentiviruses, adenoviruses and baculoviruses) for use in their specific research, and (ii) Maintain equipment for microscopy and assist in routine evaluation of cultured cells following genetic or other manipulations. Services to be added in 2008 include (i) culturing human embryonic stem cells and providing these to Liver Center Investigators for differentiation into liver cells; (ii) providing primary human liver cells isolated from resected liver segments and procured livers that are not used for transplantation; and (iii) assisting Investigators in genetically marking liver cells for identification after transplantation into animal models.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK041296-24
Application #
8500666
Study Section
Special Emphasis Panel (ZDK1-GRB-8 (J1))
Project Start
2012-06-01
Project End
2014-05-31
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
24
Fiscal Year
2012
Total Cost
$151,706
Indirect Cost
$1,002
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
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