The Cell Culture and Genetic Engineering Core has two components: I. Cell culture component: Objectives of this component are to provide Liver Research Center Investigators with: (i) isolated liver cells of consistently high quality, (iii) well-characterized frozen stocks of cell lines, (iv) specialized culture media, hormones and supplements, and matrix components for optimal cell growth;and (iv) equipment and instructions for use in isolation and culturing of various cell types derived from the liver and II. Genetic Engineering component: (i) The objectives are to assist Liver Center Investigators in developing recombinant viral vectors, (based on oncoretroviruses, lentiviruses, adenoviruses and baculoviruses) for use in their specific research, and (ii) Maintain equipment for microscopy and assist in routine evaluation of cultured cells following genetic or other manipulations. Services to be added in 2008 include (i) culturing human embryonic stem cells and providing these to Liver Center Investigators for differentiation into liver cells;(ii) providing primary human liver cells isolated from resected liver segments and procured livers that are not used for transplantation;and (iii) assisting Investigators in genetically marking liver cells for identification after transplantation into animal models.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK041296-25
Application #
8463169
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
25
Fiscal Year
2013
Total Cost
$133,379
Indirect Cost
$60,854
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Brodin, N Patrik; Chen, Yong; Yaparpalvi, Ravindra et al. (2016) Dosimetry Formalism and Implementation of a Homogenous Irradiation Protocol to Improve the Accuracy of Small Animal Whole-Body Irradiation Using a 137Cs Irradiator. Health Phys 110:S26-38
Ilyas, Ghulam; Zhao, Enpeng; Liu, Kun et al. (2016) Macrophage autophagy limits acute toxic liver injury in mice through down regulation of interleukin-1β. J Hepatol 64:118-27
Yuan, Ziqiang; Sánchez Claros, Carmen; Suzuki, Masako et al. (2016) Loss of MEN1 activates DNMT1 implicating DNA hypermethylation as a driver of MEN1 tumorigenesis. Oncotarget 7:12633-50
Taylor, Sarah A; Vittorio, Jennifer M; Martinez, Mercedes et al. (2016) Anakinra-Induced Acute Liver Failure in an Adolescent Patient with Still's Disease. Pharmacotherapy 36:e1-4
Dondossola, Eleonora; Dobroff, Andrey S; Marchiò, Serena et al. (2016) Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors. Proc Natl Acad Sci U S A 113:2223-8
Fomenko, Ekaterina Vladimirovna; Chi, Yuling (2016) Mangiferin modulation of metabolism and metabolic syndrome. Biofactors 42:492-503
Yu, Yang; Guerrero, Candace R; Liu, Shuo et al. (2016) Comprehensive Assessment of Oxidatively Induced Modifications of DNA in a Rat Model of Human Wilson's Disease. Mol Cell Proteomics 15:810-7
Merlin, Simone; Bhargava, Kuldeep K; Ranaldo, Gabriella et al. (2016) Kupffer Cell Transplantation in Mice for Elucidating Monocyte/Macrophage Biology and for Potential in Cell or Gene Therapy. Am J Pathol 186:539-51
Roy-Chowdhury, Jayanta; Schilsky, Michael L (2016) Gene therapy of Wilson disease: A ""golden"" opportunity using rAAV on the 50th anniversary of the discovery of the virus. J Hepatol 64:265-7
Kerzerho, Jerome; McIlvaine, Elizabeth J; Anthony, Patricia et al. (2016) Impact of Hepatitis C Virus on the Circulating Levels of IL-7 in HIV-1 Coinfected Women. J Acquir Immune Defic Syndr 71:172-80

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