The mission ofthe Genetic Engineering and Gene Therapy (GEGT) Core is to work cooperatively with Liver Research Center (LRC) Investigators to facilitate the development of genetic engineering reagents for in vitro and in vivo experiments.
The specific aims are to (1) generate customized oncoretroviral, lentiviral, adenoviral and non-viral vectors with integrating or episomal characteristics;(2) provide state-of-the-art reagents for site-specific gene insertion for cell marking, phenotypic correction/modification or down-regulating gene expression;(3) provide viral and non-viral vectors for reprogramming somatic cells to induced pluripotent stem (IPS) cells;(4) when appropriate, train investigators and their research personnel in generating gene transfer vectors in their own laboratories;(5) provide consultation for study design and, if appropriate, data analysis to the investigators. To fulfill these needs, the GEGT is established through a cooperative cost-sharing arrangement with the Gene Therapy Core of the Albert Einstein College of Medicine. The GEGT Core will function in close collaboration with other LRC Cores, as well as other institutional Shared Facilities, but the specialized services of the GEGT do not overlap with those offered by other Cores. The Core will benefit from the 25-yr experience of the Scientific Director, Jayanta Roy- Chowdhury, Professor of Medicine and Genetics in gene transfer in vivo and ex vivo for pathobiological and translational research, as well as the significant technological expertise of the Operations Director, Dr. Xia Wang. GEGT Core will add value to LRC investigators by enabling them to generate and use vectors and reagents that would be prohibitively expensive or technically difficult for individual laboratories to develop. It will also give LRC investigators priority for service and discounted chargeback rates. Resource sharing with other LRC Cores, as well as other institution-wide shared facilities will allow the Core to provide its services in an effective and cost-efficient manner. By supporting the multifaceted pathophysiological and translational research projects of the LRC members, as well as extramural investigators, the Core will be highly relevant to healthcare-related liver research.

Public Health Relevance

Microscopy and imaging enable liver investigators to detect and quantitate processes ranging from molecular interactions within cells to cell division in tissues. The potential for considerable insights provided by imaging approaches requires access to the latest technologies and the expertise to successfully employ them. The Imaging and Cell Structure Core provides Liver Research Investigators with state of the art instruments, analytical tools, reagents, and consultations to successfully incorporate imaging into their studies

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK041296-26
Application #
8743570
Study Section
Special Emphasis Panel (ZDK1-GRB-8 (J2))
Project Start
2014-08-15
Project End
2019-06-30
Budget Start
2014-08-15
Budget End
2015-06-30
Support Year
26
Fiscal Year
2014
Total Cost
$37,902
Indirect Cost
$15,206
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Brodin, N Patrik; Chen, Yong; Yaparpalvi, Ravindra et al. (2016) Dosimetry Formalism and Implementation of a Homogenous Irradiation Protocol to Improve the Accuracy of Small Animal Whole-Body Irradiation Using a 137Cs Irradiator. Health Phys 110:S26-38
Ilyas, Ghulam; Zhao, Enpeng; Liu, Kun et al. (2016) Macrophage autophagy limits acute toxic liver injury in mice through down regulation of interleukin-1β. J Hepatol 64:118-27
Yuan, Ziqiang; Sánchez Claros, Carmen; Suzuki, Masako et al. (2016) Loss of MEN1 activates DNMT1 implicating DNA hypermethylation as a driver of MEN1 tumorigenesis. Oncotarget 7:12633-50
Taylor, Sarah A; Vittorio, Jennifer M; Martinez, Mercedes et al. (2016) Anakinra-Induced Acute Liver Failure in an Adolescent Patient with Still's Disease. Pharmacotherapy 36:e1-4
Dondossola, Eleonora; Dobroff, Andrey S; Marchiò, Serena et al. (2016) Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors. Proc Natl Acad Sci U S A 113:2223-8
Fomenko, Ekaterina Vladimirovna; Chi, Yuling (2016) Mangiferin modulation of metabolism and metabolic syndrome. Biofactors 42:492-503
Yu, Yang; Guerrero, Candace R; Liu, Shuo et al. (2016) Comprehensive Assessment of Oxidatively Induced Modifications of DNA in a Rat Model of Human Wilson's Disease. Mol Cell Proteomics 15:810-7
Merlin, Simone; Bhargava, Kuldeep K; Ranaldo, Gabriella et al. (2016) Kupffer Cell Transplantation in Mice for Elucidating Monocyte/Macrophage Biology and for Potential in Cell or Gene Therapy. Am J Pathol 186:539-51
Roy-Chowdhury, Jayanta; Schilsky, Michael L (2016) Gene therapy of Wilson disease: A ""golden"" opportunity using rAAV on the 50th anniversary of the discovery of the virus. J Hepatol 64:265-7
Kerzerho, Jerome; McIlvaine, Elizabeth J; Anthony, Patricia et al. (2016) Impact of Hepatitis C Virus on the Circulating Levels of IL-7 in HIV-1 Coinfected Women. J Acquir Immune Defic Syndr 71:172-80

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