The Tissue Engineering and Cell Models Core (TECM) enables DDRCC investigators to address key questions relating to causality and mechanisms by providing novel and vetted experimental models that can be customized to their needs. It was established 21 months ago, replacing the Genomics and Molecular Engineering (GME) Core of the DDRCC. The reorganization of the Core was a direct result of two sequential annual needs assessments indicating strong interest by the membership for more physiologically-relevant experimental models to study digestive health and diseases. The TECM has three major components: (1) A centralized repository and facility for established and primary gut-relevant cell lines, which was originally part of the host-microbe core, but moved to the TECM to centralize all cell and tissue experimental systems, (2) A tissue engineering component that includes customizing and developing intestinal organoid technologies for specific applications needed by DDRCC users, and (3) an experimental systems component that includes in vivo models (rodent microsurgery and C. elegans), ex vivo models, and live functional assays. Inherent to these services, the TECM provides training and education, opportunities for cost-savings through bulk purchases (serum, disposables, etc), and technical expertise that saves investigators time and insure high quality of services. The TECM is inextricably tied to the other DDRCC cores. The integrated Translational Research (ITR) and the Host-Microbe (HM) cores are essential for providing cells, tissues, and patient samples for establishing the experimental models. The Tissue and Cell Analysis (TCA) core provides investigators with the means to analyze data derived from the model systems. Thus, the TECM Core has had great impact in enabling DDRCC members to advance knowledge in the thematic areas fostered by the DDRCC which focus on the study of IBD, host-microbe interactions, mucosal immunology and inflammation. In the past year, it was used by 86% of the DDRCC membership and was used in 195 peer-reviewed publications or 65% of the total (306) DDRCC-acknowledged publications during this period. The TECM has promoted interaction, collaboration, cost-sharing, and efficient resources utilization, and, at the same time, has enhanced the capabilities of our investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK042086-26
Application #
8971178
Study Section
Special Emphasis Panel (ZDK1-GRB-8 (M2))
Project Start
Project End
Budget Start
2015-12-01
Budget End
2016-11-30
Support Year
26
Fiscal Year
2016
Total Cost
$238,825
Indirect Cost
$87,670
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Amin, Ruhul; Asplin, John; Jung, Daniel et al. (2018) Reduced active transcellular intestinal oxalate secretion contributes to the pathogenesis of obesity-associated hyperoxaluria. Kidney Int 93:1098-1107
Miyoshi, Jun; Nobutani, Kentaro; Musch, Mark W et al. (2018) Time-, Sex-, and Dose-Dependent Alterations of the Gut Microbiota by Consumption of Dietary Daikenchuto (TU-100). Evid Based Complement Alternat Med 2018:7415975
Lu, Jing; Lu, Lei; Yu, Yueyue et al. (2018) Effects of Intestinal Microbiota on Brain Development in Humanized Gnotobiotic Mice. Sci Rep 8:5443
Meisel, Marlies; Hinterleitner, Reinhard; Pacis, Alain et al. (2018) Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature 557:580-584
Chen, Edmund B; Cason, Cori; Gilbert, Jack A et al. (2018) Current State of Knowledge on Implications of Gut Microbiome for Surgical Conditions. J Gastrointest Surg 22:1112-1123
Chew, Justin; Leypunskiy, Eugene; Lin, Jenny et al. (2018) High protein copy number is required to suppress stochasticity in the cyanobacterial circadian clock. Nat Commun 9:3004
Ruderman, Sarah; Eshein, Adam; Valuckaite, Vesta et al. (2018) Early increase in blood supply (EIBS) is associated with tumor risk in the Azoxymethane model of colon cancer. BMC Cancer 18:814
Dugas, Lara R; Lie, Louise; Plange-Rhule, Jacob et al. (2018) Gut microbiota, short chain fatty acids, and obesity across the epidemiologic transition: the METS-Microbiome study protocol. BMC Public Health 18:978
McIntosh, Christine M; Chen, Luqiu; Shaiber, Alon et al. (2018) Gut microbes contribute to variation in solid organ transplant outcomes in mice. Microbiome 6:96
Overstreet, A M; LaTorre, D L; Abernathy-Close, L et al. (2018) The JAK inhibitor ruxolitinib reduces inflammation in an ILC3-independent model of innate immune colitis. Mucosal Immunol 11:1454-1465

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