During the past funding period, the Cell Culture Core has continued to provide isolation of mouse and rat hepatocytes from normal and diseased livers, cell line banking, and distribution of human hepatocytes. A separately funded (through NIAAA beginning in mid-2001) Non-parenchymal Liver Cell Core provides freshly isolated and cultured normal or diseased rat Kupffer cells, hepatic stellate cells, and sinusoidal endothelial cells. Effective 9/1/04, the Cell Culture Core stopped preparing human hepatocytes but instead distributes human hepatocytes prepared by a commercial source (CellzDirect, Tucson, Az). This is because an agreement was made between the USC liver surgeons and CellzDirect, which supports the liver tissue repository at USC and provides human hepatocytes free of charge to Liver Center investigators. The Cell Culture Core has continued to provide a vital service to the Center members and has remained near steady state from the previoijs to the current grant cycle in terms of usage (number of preps, investigators and grants) and productivity (number of publications). Sixteen members and affiliated members used the Cell Culture Core from 1998 to 2003 with 47 publications. In the current cycle, 2003 to 2008, 18 full members used the Core and generated 50 publications. In particular, the Cell Culture Core has been instrumental in assisting young investigators in establishing their independent research programs (e.g. H.P. Yang, D. Han, C. Ji and Z.X. Liu).

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-8)
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University of Southern California
Los Angeles
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Rodgers, Mary A; Bowman, James W; Fujita, Hiroaki et al. (2014) The linear ubiquitin assembly complex (LUBAC) is essential for NLRP3 inflammasome activation. J Exp Med 211:1333-47
Chen, Wan-Ting; Tseng, Chun-Chih; Pfaffenbach, Kyle et al. (2014) Liver-specific knockout of GRP94 in mice disrupts cell adhesion, activates liver progenitor cells, and accelerates liver tumorigenesis. Hepatology 59:947-57
Saberi, Behnam; Ybanez, Maria D; Johnson, Heather S et al. (2014) Protein kinase C (PKC) participates in acetaminophen hepatotoxicity through c-jun-N-terminal kinase (JNK)-dependent and -independent signaling pathways. Hepatology 59:1543-54
Zhu, Genyuan; Wang, Miao; Spike, Benjamin et al. (2014) Differential requirement of GRP94 and GRP78 in mammary gland development. Sci Rep 4:5390
Pastuszka, Martha K; Wang, Xiangdong; Lock, Lye Lin et al. (2014) An amphipathic alpha-helical peptide from apolipoprotein A1 stabilizes protein polymer vesicles. J Control Release 191:15-23
Ooi, Ee Lyn; Chan, Stephanie T; Cho, Noell E et al. (2014) Novel antiviral host factor, TNK1, regulates IFN signaling through serine phosphorylation of STAT1. Proc Natl Acad Sci U S A 111:1909-14
Win, S; Than, T A; Fernandez-Checa, J C et al. (2014) JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death. Cell Death Dis 5:e989
Chen, Wan-Ting; Ha, Dat; Kanel, Gary et al. (2014) Targeted deletion of ER chaperone GRP94 in the liver results in injury, repopulation of GRP94-positive hepatocytes, and spontaneous hepatocellular carcinoma development in aged mice. Neoplasia 16:617-26
Lua, Ingrid; James, David; Wang, Jiaohong et al. (2014) Mesodermal mesenchymal cells give rise to myofibroblasts, but not epithelial cells, in mouse liver injury. Hepatology 60:311-22
Hughes, Michael W; Jiang, Ting-Xin; Lin, Sung-Jan et al. (2014) Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis. J Invest Dermatol 134:24-32

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