During the past funding period, the Cell Culture Core has continued to provide isolation of mouse and rat hepatocytes from normal and diseased livers, cell line banking, and distribution of human hepatocytes. A separately funded (through NIAAA beginning in mid-2001) Non-parenchymal Liver Cell Core provides freshly isolated and cultured normal or diseased rat Kupffer cells, hepatic stellate cells, and sinusoidal endothelial cells. Effective 9/1/04, the Cell Culture Core stopped preparing human hepatocytes but instead distributes human hepatocytes prepared by a commercial source (CellzDirect, Tucson, Az). This is because an agreement was made between the USC liver surgeons and CellzDirect, which supports the liver tissue repository at USC and provides human hepatocytes free of charge to Liver Center investigators. The Cell Culture Core has continued to provide a vital service to the Center members and has remained near steady state from the previoijs to the current grant cycle in terms of usage (number of preps, investigators and grants) and productivity (number of publications). Sixteen members and affiliated members used the Cell Culture Core from 1998 to 2003 with 47 publications. In the current cycle, 2003 to 2008, 18 full members used the Core and generated 50 publications. In particular, the Cell Culture Core has been instrumental in assisting young investigators in establishing their independent research programs (e.g. H.P. Yang, D. Han, C. Ji and Z.X. Liu).

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-8)
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University of Southern California
Los Angeles
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He, Lina; Gubbins, James; Peng, Zhechu et al. (2016) Activation of hepatic stellate cell in Pten null liver injury model. Fibrogenesis Tissue Repair 9:8
Zhu, Jiaming; Khemichian, Saro; Nghiem, Dat et al. (2016) Comparison of Demographic and Clinical Characteristics of Hispanic and Asian Chronic Hepatitis B Patients in Southern California. J Clin Gastroenterol 50:602-7
Yang, Heping; Liu, Ting; Wang, Jiaohong et al. (2016) Deregulated methionine adenosyltransferase α1, c-Myc, and Maf proteins together promote cholangiocarcinoma growth in mice and humans(‡). Hepatology 64:439-55
Flodby, Per; Kim, Yong Ho; Beard, LaMonta L et al. (2016) Knockout Mice Reveal a Major Role for Alveolar Epithelial Type I Cells in Alveolar Fluid Clearance. Am J Respir Cell Mol Biol 55:395-406
Dara, Lily; Liu, Zhang-Xu; Kaplowitz, Neil (2016) A murder mystery in the liver: who done it and how? J Clin Invest 126:4068-4071
Chen, Jian; Yao, Zhi-Xing; Chen, Jiun-Sheng et al. (2016) TGF-β/β2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome. J Clin Invest 126:527-42
Niu, Chao; Chauhan, Uday; Gargus, Matthew et al. (2016) Generation and Characterization of an Immortalized Human Esophageal Myofibroblast Line. PLoS One 11:e0153185
Dara, Lily; Kaplowitz, Neil (2016) The many faces of RIPK3: What about NASH? Hepatology 64:1411-1413
Li, Yuchang; Lua, Ingrid; French, Samuel W et al. (2016) Role of TGF-β signaling in differentiation of mesothelial cells to vitamin A-poor hepatic stellate cells in liver fibrosis. Am J Physiol Gastrointest Liver Physiol 310:G262-72
Dara, Lily; Liu, Zhang-Xu; Kaplowitz, Neil (2016) Questions and controversies: the role of necroptosis in liver disease. Cell Death Discov 2:16089

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