Abstract

Overview of the Zebrafish System. The zebrafish is now established as a viable complement and alternative to rodents for modeling human development and disease. The Zebrafish core (CORE B) has led the way in generating and collecting a unique repository of zebrafish hematopoietic mutants available for distribution to the international scientific community. The Center had a leadership role in the Trans-NIH Zebrafish Genome Initiative. CORE B has advanced the zebrafish model system by developing new tools and technology, and provided these, and related resources, to researchers who use the fish to study hematopoiesis and other processes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK049216-21
Application #
8695329
Study Section
Special Emphasis Panel (ZDK1-GRB-G)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
21
Fiscal Year
2014
Total Cost
$341,986
Indirect Cost
$142,504

  Institution

Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Vo, Linda T; Kinney, Melissa A; Liu, Xin et al. (2018) Regulation of embryonic haematopoietic multipotency by EZH1. Nature 553:506-510
Mandelbaum, Joseph; Shestopalov, Ilya A; Henderson, Rachel E et al. (2018) Zebrafish blastomere screen identifies retinoic acid suppression of MYB in adenoid cystic carcinoma. J Exp Med 215:2673-2685
Yamauchi, Takuji; Masuda, Takeshi; Canver, Matthew C et al. (2018) Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS. Cancer Cell 33:386-400.e5
Blaser, Bradley W; Zon, Leonard I (2018) Making HSCs in vitro: don't forget the hemogenic endothelium. Blood 132:1372-1378
Cesana, Marcella; Guo, Michael H; Cacchiarelli, Davide et al. (2018) A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development. Cell Stem Cell 22:575-588.e7
Canver, Matthew C; Bauer, Daniel E; Orkin, Stuart H (2017) Functional interrogation of non-coding DNA through CRISPR genome editing. Methods 121-122:118-129
Gansner, John M; Leung, Alexander D; Superdock, Michael et al. (2017) Sorting zebrafish thrombocyte lineage cells with a Cd41 monoclonal antibody enriches hematopoietic stem cell activity. Blood 129:1394-1397
Canver, Matthew C; Lessard, Samuel; Pinello, Luca et al. (2017) Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet 49:625-634
Blaser, Bradley W; Moore, Jessica L; Hagedorn, Elliott J et al. (2017) CXCR1 remodels the vascular niche to promote hematopoietic stem and progenitor cell engraftment. J Exp Med 214:1011-1027
Perlin, Julie R; Robertson, Anne L; Zon, Leonard I (2017) Efforts to enhance blood stem cell engraftment: Recent insights from zebrafish hematopoiesis. J Exp Med 214:2817-2827

Showing the most recent 10 out of 98 publications