Core C provides molecular assays for identifying, tracking, and quantifying cellular clones within complex multi-clonal mixtures. This function is designed to support basic stem cell biology, stem cell transplantation, and gene therapy research within the CCEH research base, but also among a broader community of basic and clinical scientists, at the FHCRC, UW, and other institutions. Services provided include VNTR analysis (Variable Number Tandem Repeat), to track the level of donor contribution in a transplant setting and LAM-PCR (Linear Amplification-Mediated PCR) to identify and track specific clones of cells in a chimeric mixture. Techniques are in place to estimate the level of gene transfer into stem cells, to estimate the number of stem cells engrafting, to detect the recruitment and exhaustion of individual stem cell clones, to identify leukemic outgrowth, and to determine insertion site specificity of retroviral vectors. A third assay category. Allele Tracking, has been added more recently to assist in the validation of transgenic and knockout mice developed by our users. To this end, we have developed nine multiplex PCR assays to quickly track the genotypes of several conditional knockout lines. Additional assays are developed a needed.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-G)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Fred Hutchinson Cancer Research Center
United States
Zip Code
Busch, S E; Moser, R D; Gurley, K E et al. (2014) ARF inhibits the growth and malignant progression of non-small-cell lung carcinoma. Oncogene 33:2665-73
Watts, Korashon L; Beard, Brian C; Wood, Brent L et al. (2014) No evidence of clonal dominance after transplant of HOXB4-expanded cord blood cells in a nonhuman primate model. Exp Hematol 42:497-504
Klippel, Z K; Chou, J; Towlerton, A M et al. (2014) Immune escape from NY-ESO-1-specific T-cell therapy via loss of heterozygosity in the MHC. Gene Ther 21:337-42
Wurm, Melanie; Kowalski, John; Heckl, Dirk et al. (2014) Ectopic expression of HOXC6 blocks myeloid differentiation and predisposes to malignant transformation. Exp Hematol 42:114-25.e4
Adair, Jennifer E; Johnston, Sandra K; Mrugala, Maciej M et al. (2014) Gene therapy enhances chemotherapy tolerance and efficacy in glioblastoma patients. J Clin Invest 124:4082-92
Iwata, Mineo; Sandstrom, Richard S; Delrow, Jeffrey J et al. (2014) Functionally and phenotypically distinct subpopulations of marrow stromal cells are fibroblast in origin and induce different fates in peripheral blood monocytes. Stem Cells Dev 23:729-40
Green, Damian J; Orgun, Nural N; Jones, Jon C et al. (2014) A preclinical model of CD38-pretargeted radioimmunotherapy for plasma cell malignancies. Cancer Res 74:1179-89
Burtner, Christopher R; Beard, Brian C; Kennedy, Douglas R et al. (2014) Intravenous injection of a foamy virus vector to correct canine SCID-X1. Blood 123:3578-84
Bleakley, Marie; Heimfeld, Shelly; Jones, Lori A et al. (2014) Engineering human peripheral blood stem cell grafts that are depleted of naïve T cells and retain functional pathogen-specific memory T cells. Biol Blood Marrow Transplant 20:705-16
Shu, Z; Heimfeld, S; Gao, D (2014) Hematopoietic SCT with cryopreserved grafts: adverse reactions after transplantation and cryoprotectant removal before infusion. Bone Marrow Transplant 49:469-76

Showing the most recent 10 out of 175 publications