Core C provides molecular assays for identifying, tracking, and quantifying cellular clones within complex multi-clonal mixtures. This function is designed to support basic stem cell biology, stem cell transplantation, and gene therapy research within the CCEH research base, but also among a broader community of basic and clinical scientists, at the FHCRC, UW, and other institutions. Services provided include VNTR analysis (Variable Number Tandem Repeat), to track the level of donor contribution in a transplant setting and LAM-PCR (Linear Amplification-Mediated PCR) to identify and track specific clones of cells in a chimeric mixture. Techniques are in place to estimate the level of gene transfer into stem cells, to estimate the number of stem cells engrafting, to detect the recruitment and exhaustion of individual stem cell clones, to identify leukemic outgrowth, and to determine insertion site specificity of retroviral vectors. A third assay category. Allele Tracking, has been added more recently to assist in the validation of transgenic and knockout mice developed by our users. To this end, we have developed nine multiplex PCR assays to quickly track the genotypes of several conditional knockout lines. Additional assays are developed a needed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK056465-16
Application #
8698730
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
16
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
City
Seattle
State
WA
Country
United States
Zip Code
98109
OhAinle, Molly; Helms, Louisa; Vermeire, Jolien et al. (2018) A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. Elife 7:
Sorror, Mohamed L; Gooley, Ted A; Maclean, Kirsteen H et al. (2018) Pre-transplant expressions of microRNAs, comorbidities, and post-transplant mortality. Bone Marrow Transplant :
Gauthier, Jordan; Turtle, Cameron J (2018) Insights into cytokine release syndrome and neurotoxicity after CD19-specific CAR-T cell therapy. Curr Res Transl Med 66:50-52
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Roesch, Ferdinand; OhAinle, Molly; Emerman, Michael (2018) A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery. Retrovirology 15:26
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