Abstract

Analysis of individual cells and cell populations at the spatial, temporal and functional level lies at the heart of modern immunology, cell biology and developmental biology. The Cytometry Core focuses on the provision of light microscopal or fluorescence activated cell sorting (FACS) technologies which can be applied in combination with specific molecular probes such as antibodies, DNA, MHC-tetramers, intracellular reporter molecules to address fundamental questions relating to the pathogenesis, treatment and complications of type 1 and 2 diabetes. The Core will be located at the Barbara Davis Center and manned by 2 FTE technical staff under the director ship of Dr. D. Bellgrau. It will use items of large equipment recently obtained from NIH and charitable funding. Twelve services will be offered including standard FACS analyses and immunohistochemistry of solid tissues and more sophisticated analyses of signal transduction and subcellular events in living cells. Formal training will be offered without charge on a biweekly to monthly basis and expert consultation will be available for each of the services provided.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
3P30DK057516-02S1
Application #
6503600
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2001-04-01
Project End
2002-03-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Millstein, Richard J; Pyle, Laura L; Bergman, Bryan C et al. (2018) Sex-specific differences in insulin resistance in type 1 diabetes: The CACTI cohort. J Diabetes Complications 32:418-423
Steck, Andrea K; Dong, Fran; Frohnert, Brigitte I et al. (2018) Predicting progression to diabetes in islet autoantibody positive children. J Autoimmun 90:59-63
Costacou, Tina; Crandell, Jamie; Kahkoska, Anna R et al. (2018) Dietary Patterns Over Time and Microalbuminuria in Youth and Young Adults With Type 1 Diabetes: The SEARCH Nutrition Ancillary Study. Diabetes Care 41:1615-1622
Jaiswal, Mamta; Divers, Jasmin; Urbina, Elaine M et al. (2018) Cardiovascular autonomic neuropathy in adolescents and young adults with type 1 and type 2 diabetes: The SEARCH for Diabetes in Youth Cohort Study. Pediatr Diabetes 19:680-689
Liu, Chih-Wei; Bramer, Lisa; Webb-Robertson, Bobbie-Jo et al. (2018) Temporal expression profiling of plasma proteins reveals oxidative stress in early stages of Type 1 Diabetes progression. J Proteomics 172:100-110
Reynolds, Kristi; Saydah, Sharon H; Isom, Scott et al. (2018) Mortality in youth-onset type 1 and type 2 diabetes: The SEARCH for Diabetes in Youth study. J Diabetes Complications 32:545-549
Plenter, Robert J; Grazia, Todd J; Coulombe, Marilyne G et al. (2018) Anti-LFA-1 induces CD8 T-cell dependent allograft tolerance and augments suppressor phenotype CD8 cells. Cell Immunol 332:101-110
Liese, Angela D; Ma, Xiaonan; Ma, Xiaoguang et al. (2018) Dietary quality and markers of inflammation: No association in youth with type 1 diabetes. J Diabetes Complications 32:179-184
Kahkoska, Anna R; Shay, Christina M; Couch, Sarah C et al. (2018) Sociodemographic associations of longitudinal adiposity in youth with type 1 diabetes. Pediatr Diabetes 19:1429-1440
Lee-Sherick, Alisa B; Jacobsen, Kristen M; Henry, Curtis J et al. (2018) MERTK inhibition alters the PD-1 axis and promotes anti-leukemia immunity. JCI Insight 3:

Showing the most recent 10 out of 480 publications