Statement of work The Broad Institute will support the work of BADERC investigators through engagement of the following Broad Platforms: Metabolite Profiling, Proteomics, RNAi and Genetic Analysis. We propose two modes in which BADERC investigators can utilize this support: First is through the pre-existing Pilot and Feasibility process. We envision that a number of these P&F proposals will aim to incorporate Broad platform capabilities in their proposals (for example, a project that performs metabolic profiling and / or RNAi screening capabilities, or that utilizes SNP genotyping in a human genetic aims with the Genetic Analysis Platform). The Project Manager (Ms. Burtt) and PI (Altshuler) will help investigators identify and engage these opportunities, to interact with platform staff to assess feasibility and details of experimental design, and to write proposals utilizing these platforms. If these P&F proposals are judged meritorious and selected for funding (by the existing BADERC mechanism), then the costs incurred by the Broad platforms would be supported by the budgeted funds. The program manager would then facilitate the execution of the approved project activities. Second, there are a number of platform capabilities that will be available to BADERC investigators as needed based on their existing research. Examples might include SNP genotyping for an ongoing project that tests whether a novel candidate gene contributes to a diabetes-related phenotype in humans, or creation of new RNAi reagents to perform knock-down assays in a cellular diabetes model. The Broad subcontract will support access to such Broad platform capabilities by each BADERC investigator (up to a pre-specified limit per investigator, not to exceed the total budgeted). As above, the project manager would serve as a key liaison between BADERC investigators and Broad Platforms, helping identify which capabilities matched each funding mechanism, guiding investigators to the relevant staff, and facilitating successful execution of each approved activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK057521-13
Application #
8378793
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
13
Fiscal Year
2012
Total Cost
$126,980
Indirect Cost
$20,223
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Kim, MiSung; Astapova, Inna I; Flier, Sarah N et al. (2017) Intestinal, but not hepatic, ChREBP is required for fructose tolerance. JCI Insight 2:
Mumtaz, Rizwan; Trepiccione, Francesco; Hennings, J Christopher et al. (2017) Intercalated Cell Depletion and Vacuolar H+-ATPase Mistargeting in an Ae1 R607H Knockin Model. J Am Soc Nephrol 28:1507-1520
Palmer, Colin J; Bruckner, Raphael J; Paulo, Joao A et al. (2017) Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. Mol Metab 6:1212-1225
Dai, Ning; Ji, Fei; Wright, Jason et al. (2017) IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1. Elife 6:
Fenselau, Henning; Campbell, John N; Verstegen, Anne M J et al. (2017) A rapidly acting glutamatergic ARC?PVH satiety circuit postsynaptically regulated by ?-MSH. Nat Neurosci 20:42-51
Kitano, Kentaro; Schwartz, Dana M; Zhou, Haiyang et al. (2017) Bioengineering of functional human induced pluripotent stem cell-derived intestinal grafts. Nat Commun 8:765
Li, Wei; Jin, William W; Tsuji, Kenji et al. (2017) Ezrin directly interacts with AQP2 and promotes its endocytosis. J Cell Sci 130:2914-2925
Cheng, Longzhen; Duan, Bo; Huang, Tianwen et al. (2017) Identification of spinal circuits involved in touch-evoked dynamic mechanical pain. Nat Neurosci 20:804-814
Fisher, Ffolliott M; Kim, MiSung; Doridot, Ludivine et al. (2017) A critical role for ChREBP-mediated FGF21 secretion in hepatic fructose metabolism. Mol Metab 6:14-21
Campbell, John N; Macosko, Evan Z; Fenselau, Henning et al. (2017) A molecular census of arcuate hypothalamus and median eminence cell types. Nat Neurosci 20:484-496

Showing the most recent 10 out of 372 publications