The main objectives of the Preclinical Models of Digestive Diseases Core are to provide VDDRC investigators with access to non-invasive small animal imaging, novel conditionally immortalized murine gastrointestinal epithelial cell lines, state-of-the art research immunohistochemistry, and consultative services in interpretation of morphologic findings in animal models and human digestive diseases. The core has three components: the Preclinical Imaging Subcore, the Novel Cell Line Development Subcore and the Tissue Morphology Subcore. The expansion and modification of this Core now provides VDDRC investigators the opportunity to move nimbly from investigations utilizing non-invasive molecular imaging (Preclinical Imaging Subcore) and histology (Tissue Morphology Subcore), often in tandem, in tractable transgenic model systems of digestive diseases (Novel Ceil Lines Subcore) to mechanistic studies using novel cell culture-based systems. In vitro studies that employ conditionally immortalized transgenic cell lines will subsequently inform and guide new in vivo experimentation which will ultimately culminate in studies of clinical populations. New services will include access to a variety of molecular imaging probes specifically tailored for imaging GI disease in preclinical animal models, including NIR-based optical imaging reagents and translational imaging compounds labeled with positron emitting isotopes (PET). The Preclinical imaging Subcore will support all major small animal imaging modalities including optical, SPECT, and PET methods for molecular imaging, as provided by the Center for Small Animal Imaging within the Vanderbilt University institute of Imaging Science. The Novel Cell Line Development Subcore provides a unique service in developing conditionally immortalized epithelial cell lines from the different segments of the gastrointestinal tract of transgenic mice with gene deletions known to be associated with known gastrointestinal pathologies. This subcore addresses the lack of normal epithelial cell lines derived from intestinal mucosa by utilizing a novel transgenic mouse, the """"""""Immortomouse,"""""""" which carries a temperature-sensitive mutant of the SV40 large T gene, an immortalizing gene active only at the permissive temperature. The Tissue Morphology Subcore provides research immunohistochemistry services for both human and animal intestinal tissue. In addition, Dr. Washington, the core Director, provides the necessary expertise for interpretation of the histologic findings obtained in these studies.

Public Health Relevance

This core provides techniques and tools applicable to the study of many different digestive diseases. These services include special cell lines custom-tailored to the relevant disease, small animal radiology, and expert examination of tissue samples by a pathologist specializing in digestive diseases.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Vanderbilt University Medical Center
United States
Zip Code
Chen, Zheng; Hu, Tianling; Zhu, Shoumin et al. (2017) Glutathione peroxidase 7 suppresses cancer cell growth and is hypermethylated in gastric cancer. Oncotarget 8:54345-54356
Petersen, Christine P; Meyer, Anne R; De Salvo, Carlo et al. (2017) A signalling cascade of IL-33 to IL-13 regulates metaplasia in the mouse stomach. Gut :
Wang, Yang; Shi, Chanjuan; Eisenberg, Rosana et al. (2017) Differences in Microsatellite Instability Profiles between Endometrioid and Colorectal Cancers: A Potential Cause for False-Negative Results? J Mol Diagn 19:57-64
Noto, Jennifer M; Peek Jr, Richard M (2017) Helicobacter pylori Makes a Molecular Incision to Gain Epithelial Entry. Cell Host Microbe 22:434-436
Messaggio, Fanuel; Mendonsa, Alisha M; Castellanos, Jason et al. (2017) Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth. Oncotarget 8:85378-85391
Stephenson, Jason R; Wang, Xiaohan; Perfitt, Tyler L et al. (2017) A Novel Human CAMK2A Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. J Neurosci 37:2216-2233
Nicholas, Katherine J; Flaherty, David K; Smith, Rita M et al. (2017) Chronic HIV-1 Infection Impairs Superantigen-Induced Activation of Peripheral CD4+CXCR5+PD-1+ Cells, With Relative Preservation of Recall Antigen-Specific Responses. J Acquir Immune Defic Syndr 74:72-80
Pilkinton, Mark A; Nicholas, Katherine J; Warren, Christian M et al. (2017) Greater activation of peripheral T follicular helper cells following high dose influenza vaccine in older adults forecasts seroconversion. Vaccine 35:329-336
LePage, Daniel P; Metcalf, Jason A; Bordenstein, Sarah R et al. (2017) Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility. Nature 543:243-247
Hardbower, D M; Coburn, L A; Asim, M et al. (2017) EGFR-mediated macrophage activation promotes colitis-associated tumorigenesis. Oncogene 36:3807-3819

Showing the most recent 10 out of 1230 publications