It is clear that the progress of diabetes research during the coming decade will depend heavily upon the ability to ufilize the mouse as an experimental model to invesfigate both basic and clinically relevant quesfions in diabetes research. The Transgenic and Knock-out Mouse Core (TKMC, Core A) provides invesfigators at UCLA, UCSD, the Salk Institute, and Cedars-Sinai with a wide array of genefic manipulafions in the mouse including transgenic genes, homologous recombinafion in embryonic stem cells (ES cells), creation of chimeric mice from ES cells, and the most cutting-edge approaches to performing reverse genefics in the mouse. Transgenic, knock-out and knock-in mouse models are created that utilize the most advanced approaches including condifional Tet-inducible and tamoxifen-inducible transgenes, fissue-specific and condifional knock-outs using Cre-LoxP and Flp recombinases and recombinafion-mediated cassette exchange (RMCE), BAC transgenics, BAC-Trap, RiboTag, and other specialized technologies. This Core is an outstanding example of how extraordinarily specialized techniques, highly trained dedicated personnel, specially constructed facilities, and expensive equipment can be accessed by researchers who could not reasonably expect to develop them on an individual basis. Key objectives are: 1. To create innovative and important mouse models for studies of diabetes and its complicafions 2. To eliminate barriers to the most cutfing-edge mouse genefic approaches for the DERC membership 3. To provide outstanding, reliable, and high quality mouse embryology and genefic services 4. To advance the technology of genefic manipulafion of the mouse genome The availability of this Transgenic and Knock-out Mouse Core in coordinafion with the Metabolic and Molecular Physiology Core, the Genomics and Epigenefics Core, and the Novel Target idenfificafion and Assay Development Core, will enable our members to conduct versafile, cutting-edge, reverse genefic research in the mouse with a battery of multidisciplinary, state-of-the-art techniques.
This Core provides services allowing the creation of sophisticated mouse models for the DRC Membership to address the mechanisms of diabetes and other endocrine diseases. The strong conservation between the genomes of humans and mice makes the approach of using transgenic and knock-out mouse technology to create models for human diabetes, endocrine pathologies, and diabetes complications extremely useful.
|Mahajan, Anubha (see original citation for additional authors) (2018) Refining the accuracy of validated target identification through coding variant fine-mapping in type 2 diabetes. Nat Genet 50:559-571|
|Hajek, Catherine; Guo, Xiuqing; Yao, Jie et al. (2018) Coronary Heart Disease Genetic Risk Score Predicts Cardiovascular Disease Risk in Men, Not Women. Circ Genom Precis Med 11:e002324|
|Malik, Rainer (see original citation for additional authors) (2018) Multiancestry genome-wide association study of 520,000 subjects identifies 32 loci associated with stroke and stroke subtypes. Nat Genet 50:524-537|
|Kulminski, Alexander M; Huang, Jian; Loika, Yury et al. (2018) Strong impact of natural-selection-free heterogeneity in genetics of age-related phenotypes. Aging (Albany NY) 10:492-514|
|Gao, Chuan; Tabb, Keri L; Dimitrov, Latchezar M et al. (2018) Exome Sequencing Identifies Genetic Variants Associated with Circulating Lipid Levels in Mexican Americans: The Insulin Resistance Atherosclerosis Family Study (IRASFS). Sci Rep 8:5603|
|Seyerle, A A; Sitlani, C M; Noordam, R et al. (2018) Pharmacogenomics study of thiazide diuretics and QT interval in multi-ethnic populations: the cohorts for heart and aging research in genomic epidemiology. Pharmacogenomics J 18:215-226|
|Yuan, Xiaomei; Dong, Yi; Tsurushita, Naoya et al. (2018) CD122 blockade restores immunological tolerance in autoimmune type 1 diabetes via multiple mechanisms. JCI Insight 3:|
|Svensson, Kristoffer; Dent, Jess R; Tahvilian, Shahriar et al. (2018) Defining the contribution of skeletal muscle pyruvate dehydrogenase alpha 1 (Pdha1) to exercise performance and insulin action. Am J Physiol Endocrinol Metab :|
|Wang, Bo; Rong, Xin; Palladino, Elisa N D et al. (2018) Phospholipid Remodeling and Cholesterol Availability Regulate Intestinal Stemness and Tumorigenesis. Cell Stem Cell 22:206-220.e4|
|Gong, J; Nishimura, K K; Fernandez-Rhodes, L et al. (2018) Trans-ethnic analysis of metabochip data identifies two new loci associated with BMI. Int J Obes (Lond) 42:384-390|
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