It is clear that the progress of diabetes research during the coming decade will depend heavily upon the ability to ufilize the mouse as an experimental model to invesfigate both basic and clinically relevant quesfions in diabetes research. The Transgenic and Knock-out Mouse Core (TKMC, Core A) provides invesfigators at UCLA, UCSD, the Salk Institute, and Cedars-Sinai with a wide array of genefic manipulafions in the mouse including transgenic genes, homologous recombinafion in embryonic stem cells (ES cells), creation of chimeric mice from ES cells, and the most cutting-edge approaches to performing reverse genefics in the mouse. Transgenic, knock-out and knock-in mouse models are created that utilize the most advanced approaches including condifional Tet-inducible and tamoxifen-inducible transgenes, fissue-specific and condifional knock-outs using Cre-LoxP and Flp recombinases and recombinafion-mediated cassette exchange (RMCE), BAC transgenics, BAC-Trap, RiboTag, and other specialized technologies. This Core is an outstanding example of how extraordinarily specialized techniques, highly trained dedicated personnel, specially constructed facilities, and expensive equipment can be accessed by researchers who could not reasonably expect to develop them on an individual basis. Key objectives are: 1. To create innovative and important mouse models for studies of diabetes and its complicafions 2. To eliminate barriers to the most cutfing-edge mouse genefic approaches for the DERC membership 3. To provide outstanding, reliable, and high quality mouse embryology and genefic services 4. To advance the technology of genefic manipulafion of the mouse genome The availability of this Transgenic and Knock-out Mouse Core in coordinafion with the Metabolic and Molecular Physiology Core, the Genomics and Epigenefics Core, and the Novel Target idenfificafion and Assay Development Core, will enable our members to conduct versafile, cutting-edge, reverse genefic research in the mouse with a battery of multidisciplinary, state-of-the-art techniques.

Public Health Relevance

This Core provides services allowing the creation of sophisticated mouse models for the DRC Membership to address the mechanisms of diabetes and other endocrine diseases. The strong conservation between the genomes of humans and mice makes the approach of using transgenic and knock-out mouse technology to create models for human diabetes, endocrine pathologies, and diabetes complications extremely useful.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California San Diego
La Jolla
United States
Zip Code
Sen, Supriya; Langiewicz, Magda; Jumaa, Hassan et al. (2015) Deletion of serine/arginine-rich splicing factor 3 in hepatocytes predisposes to hepatocellular carcinoma in mice. Hepatology 61:171-83
Chung, H; Lee, Y S; Mayoral, R et al. (2015) Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer. Oncogene 34:3504-13
Adar, Sara D; Kaufman, Joel D; Diez-Roux, Ana V et al. (2015) Air pollution and percent emphysema identified by computed tomography in the Multi-Ethnic study of Atherosclerosis. Environ Health Perspect 123:144-51
Weizman, Adam; Huang, Brian; Berel, Dror et al. (2014) Clinical, serologic, and genetic factors associated with pyoderma gangrenosum and erythema nodosum in inflammatory bowel disease patients. Inflamm Bowel Dis 20:525-33
Baker, Michael E; Hardiman, Gary (2014) Transcriptional analysis of endocrine disruption using zebrafish and massively parallel sequencing. J Mol Endocrinol 52:R241-56
Huang, Jie; Huffman, Jennifer E; Yamakuchi, Munekazu et al. (2014) Genome-wide association study for circulating tissue plasminogen activator levels and functional follow-up implicates endothelial STXBP5 and STX2. Arterioscler Thromb Vasc Biol 34:1093-101
Bis, Joshua C; White, Charles C; Franceschini, Nora et al. (2014) Sequencing of 2 subclinical atherosclerosis candidate regions in 3669 individuals: Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Consortium Targeted Sequencing Study. Circ Cardiovasc Genet 7:359-64
Tang, Wenbo; Kowgier, Matthew; Loth, Daan W et al. (2014) Large-scale genome-wide association studies and meta-analyses of longitudinal change in adult lung function. PLoS One 9:e100776
Lubitz, Steven A; Lunetta, Kathryn L; Lin, Honghuang et al. (2014) Novel genetic markers associate with atrial fibrillation risk in Europeans and Japanese. J Am Coll Cardiol 63:1200-10
Nalls, Mike A; Pankratz, Nathan; Lill, Christina M et al. (2014) Large-scale meta-analysis of genome-wide association data identifies six new risk loci for Parkinson's disease. Nat Genet 46:989-93

Showing the most recent 10 out of 323 publications