The Histopathology Core provides a wide spectrum of histology and microscopy services to meet the growing needs of diabetes researchers at Columbia University and affiliated institutions. Over the previous funding cycle, due to ovenwhelming demand for these essential services, the histopathology laboratory (formerly part of the Phenotyping core) and microscopy services (formeriy part of the Genomics core) have been consolidated into a new Histopathology Core. The services of this new core include the high quality basic histological and microscopy services offered in the previous funding cycle, but have been expanded to accommodate the changing and growing needs of DRC users and to incorporate newly developed reagents and technologies. Furthermore, Dr. Sussel, a leader in islet biology and pancreas development was recruited to Columbia University and the Berrie Diabetes Center in 2008 and was enlisted to develop and direct this new core. Since its establishment in 2008, the Histopathology Core has assisted 32 DRC investigators supported by 69 grants and 6 non-DRC investigators on projects that have resulted in 61 papers (29 as primary Core) and/or provided data that helped investigators obtain 14 new grants. It has processed 30,000 samples, cut -300,000 sections and performed 36,000 immunohistochemical stains, nearly 18,000 of which by immunofluorescence. The Core plans to continue to provide advanced histophenotyping capabilities to the thriving Columbia University diabetes research community. To this end, the Core will seamlessly integrated its operation with that of the other DRC Core facilities, to maintain an investigator-oriented approach to service and development, implement and sustain effective business practices, and assist the DRC in its overall mission.
Tissue phenotyping is an essential component of analytical tools to understand, diagnose, and treat diabetes. The purpose of this facility is to ease access to complex, time-consuming, and technically intensive procedures to assist DRC investigators, accelerate discovery, streamline practices, and reduce costs and animal use.
|Granot, Tomer; Senda, Takashi; Carpenter, Dustin J et al. (2017) Dendritic Cells Display Subset and Tissue-Specific Maturation Dynamics over Human Life. Immunity 46:504-515|
|Dastagir, Shamael R; Postigo-Fernandez, Jorge; Xu, Chunliang et al. (2017) Efficient Presentation of Multiple Endogenous Epitopes to Both CD4+ and CD8+ Diabetogenic T Cells for Tolerance. Mol Ther Methods Clin Dev 4:27-38|
|Holter, Marlena M; Dutia, Roxanne; Stano, Sarah M et al. (2017) Glucose Metabolism After Gastric Banding and Gastric Bypass in Individuals With Type 2 Diabetes: Weight Loss Effect. Diabetes Care 40:7-15|
|Wang, Ying; Subramanian, Manikandan; Yurdagul Jr, Arif et al. (2017) Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by Macrophages. Cell 171:331-345.e22|
|Kumar, Brahma V; Ma, Wenji; Miron, Michelle et al. (2017) Human Tissue-Resident Memory T Cells Are Defined by Core Transcriptional and Functional Signatures in Lymphoid and Mucosal Sites. Cell Rep 20:2921-2934|
|Demmer, Ryan T; Breskin, Alexander; Rosenbaum, Michael et al. (2017) The subgingival microbiome, systemic inflammation and insulin resistance: The Oral Infections, Glucose Intolerance and Insulin Resistance Study. J Clin Periodontol 44:255-265|
|Ishida, Emi; Kim-Muller, Ja Young; Accili, Domenico (2017) Pair Feeding, but Not Insulin, Phloridzin, or Rosiglitazone Treatment, Curtails Markers of ?-Cell Dedifferentiation in db/db Mice. Diabetes 66:2092-2101|
|Gutiérrez, Giselle Domínguez; Bender, Aaron S; Cirulli, Vincenzo et al. (2017) Pancreatic ? cell identity requires continual repression of non-? cell programs. J Clin Invest 127:244-259|
|Morabito, Michael V; Ravussin, Yann; Mueller, Bridget R et al. (2017) Weight Perturbation Alters Leptin Signal Transduction in a Region-Specific Manner throughout the Brain. PLoS One 12:e0168226|
|Stano, Sarah; Alam, Fatima; Wu, Louis et al. (2017) Effect of meal size and texture on gastric pouch emptying and glucagon-like peptide 1 after gastric bypass surgery. Surg Obes Relat Dis 13:1975-1983|
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