The Flow Cytometry Core has been established during the past year at the Columbia Diabetes Research Center. In the past funding cycle, there has been substantial growth in requests for flow cytometry services to assist diabetes investigators. The interest has been catalyzed by several initiatives to study pathogenesis and treatment of Type 1 Diabetes, including the new NIDDK-funded Epigenetics Program, the creation ofthe Columbia Center for Translational Immunology, and Genetics of autoimmunity initiative. In addition, the ability to isolate genetically modified rare cell populations from organs like brain, endocrine pancreas, adipose tissue, and gut has become critical to the research of DRC faculty. The DRC Flow Core was established in 2011 with substantial institutional as well as NIH support in the form of a Shared Instrumentation Grant. It was fully integrated within the DRC during the past year to meet the growing need ofthe DRC User base for flow cytometry, as demonstrated by 17 users supported by 33 grants, who have already obtained 5 new grants. The Core has already supported 16 publications as primary Core. User projections demonstrate a growing demand for these services, with 37 declared users for the coming year. The expertly staffed Flow Core is situated in renovated Department of Medicine space and houses four, state-of-the-art BD flow cytometers, including a 6-laser LSR II and a 4-laser BD Influx sorter. There is strong demand for these high-quality services. In particular, the BD Influx is only the second biosafety level-2 sorter available campus-wide for human cell sorting. More than 90% ofthe current use is by DRC faculty, a substantial portion of which are new NIH-funded Investigators recruited to CUMC in the previous funded period (Sykes, Farber, Yang). The Core Director Dr. Clynes is thoroughly integrated within the DRC and the Columbia biomedical community, and its Technical Director Dr. Siu Hong Ho is a superb operator and immunologist, enabling this Core to be a vibrant collaborative center of excellence.

Public Health Relevance

The identification and isolation of high quality, living cellular populations that occur at low frequency in different organ and tissue types is critical to the research endeavors ofthe Columbia diabetes community. The availability of this shared Core Facilities enables individual investigators to have access to expensive equipment and technically demanding methods that would not otherwise be affordable.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-S (O2))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Columbia University (N.Y.)
New York
United States
Zip Code
Savage, Thomas M; Shonts, Brittany A; Obradovic, Aleksandar et al. (2018) Early expansion of donor-specific Tregs in tolerant kidney transplant recipients. JCI Insight 3:
Molusky, Matthew M; Hsieh, Joanne; Lee, Samuel X et al. (2018) Metformin and AMP Kinase Activation Increase Expression of the Sterol Transporters ABCG5/8 (ATP-Binding Cassette Transporter G5/G8) With Potential Antiatherogenic Consequences. Arterioscler Thromb Vasc Biol 38:1493-1503
Carpenter, D J; Granot, T; Matsuoka, N et al. (2018) Human immunology studies using organ donors: Impact of clinical variations on immune parameters in tissues and circulation. Am J Transplant 18:74-88
Langlet, Fanny; Tarbier, Marcel; Haeusler, Rebecca A et al. (2018) microRNA-205-5p is a modulator of insulin sensitivity that inhibits FOXO function. Mol Metab 17:49-60
Proto, Jonathan D; Doran, Amanda C; Gusarova, Galina et al. (2018) Regulatory T Cells Promote Macrophage Efferocytosis during Inflammation Resolution. Immunity 49:666-677.e6
Carli, Jayne F Martin; LeDuc, Charles A; Zhang, Yiying et al. (2018) The role of Rpgrip1l, a component of the primary cilium, in adipocyte development and function. FASEB J 32:3946-3956
Postigo-Fernandez, Jorge; Creusot, RĂ©mi J (2018) A multi-epitope DNA vaccine enables a broad engagement of diabetogenic T cells for tolerance in Type 1 diabetes. J Autoimmun :
Proto, Jonathan D; Doran, Amanda C; Subramanian, Manikandan et al. (2018) Hypercholesterolemia induces T cell expansion in humanized immune mice. J Clin Invest 128:2370-2375
Martin Carli, Jayne F; LeDuc, Charles A; Zhang, Yiying et al. (2018) FTO mediates cell-autonomous effects on adipogenesis and adipocyte lipid content by regulating gene expression via 6mA DNA modifications. J Lipid Res 59:1446-1460
Arnes, Luis; Liu, Zhaoqi; Wang, Jiguang et al. (2018) Comprehensive characterisation of compartment-specific long non-coding RNAs associated with pancreatic ductal adenocarcinoma. Gut :

Showing the most recent 10 out of 225 publications