Rodent models of diabetes and its complications have and v /ill continue to provide fundamental insights into the molecular basis of human metabolic disease. The investigators of the Columbia DRC employ more than one hundred murine models to study diabetes and its complications. Characterizing the phenotype of these animals in an efficient, effective and timely fashion is critical for the productive study of diabetes at Columbia. The Mouse Phenotyping Core (MPC) has been in great demand during the current funding cycle and has evolved significantly since the last submission to meet the needs of DRC investigators. The MPC has proven broadly successful in achieving its mission of assisting individual investigators in characterizing metabolic phenotypes of mice, fulfilling more than 23,000 service requests for 17 DRC members supported by 42 grants, and for 11 non-DRC members. The Core has been instrumental in obtaining 11 new grants, and in 60 publications (32 as primary Core). The Mouse Phenotyping Core currently provides services that facilitate the efficient characterization of mouse models of diabetes and its complications: Body Composition Analysis, Whole Body Metabolic Assessment and Multiplex Protein Assays. The MPC services complement those provided by other DRC Cores, so that investigators who take advantage of DRC resources can fully characterize mice with genetic, histologic, immunologic and metabolic analyses. The Core has been built through the strong support ofthe Naomi Berrie Diabetes Center and Columbia University. To continue effectively serving the needs ofthe Columbia diabetes research community, the Mouse Phenotyping Core has evolved by adding and expanding services. In response to investigator surveys and as proposed in our last competing renewal, we developed two phenotyping services during the current funding period: Whole Body Metabolic Assessment and Lipidomics. These services are now well established and indeed have been in such high demand and so successful that they have each undergone one round of expansion. In response to a more recent set of DRC surveys and investigator requests, we have completed the expansion of the Metabolic Clamp Assessment. Projections indicate that demand for these Core services will remain high, should we be refunded.

Public Health Relevance

The ability to perform advanced metabolic phenotyping of rodent models of diabetes, obesity, and insulin resistance, is essential to the operation of a modern Diabetes Research Center. The Mouse Phenotyping Core has fulfilled this mission by offering high-quality in vivo characterization of whole body metabolism and selected metabolites. With the implementation of glucose clamps, this Core is poised to continue to provide critical support to DRC Activities.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Columbia University (N.Y.)
New York
United States
Zip Code
Kode, A; Mosialou, I; Manavalan, S J et al. (2016) FoxO1-dependent induction of acute myeloid leukemia by osteoblasts in mice. Leukemia 30:1-13
Kim-Muller, Ja Young; Kim, Young Jung R; Fan, Jason et al. (2016) FoxO1 Deacetylation Decreases Fatty Acid Oxidation in β-Cells and Sustains Insulin Secretion in Diabetes. J Biol Chem 291:10162-72
Kuo, Taiyi; Kim-Muller, Ja Young; McGraw, Timothy E et al. (2016) Altered Plasma Profile of Antioxidant Proteins as an Early Correlate of Pancreatic β Cell Dysfunction. J Biol Chem 291:9648-56
Lerea, Jaclyn S; Ring, Laurence E; Hassouna, Rim et al. (2016) Reducing Adiposity in a Critical Developmental Window Has Lasting Benefits in Mice. Endocrinology 157:666-78
Xuan, Shouhong; Sussel, Lori (2016) GATA4 and GATA6 regulate pancreatic endoderm identity through inhibition of hedgehog signaling. Development 143:780-6
Grijalva, Ambar; Xu, Xiaoyuan; Ferrante Jr, Anthony W (2016) Autophagy Is Dispensable for Macrophage-Mediated Lipid Homeostasis in Adipose Tissue. Diabetes 65:967-80
Juan De Solis, Alain; Baquero, Arian F; Bennett, Camdin M et al. (2016) Postnatal undernutrition delays a key step in the maturation of hypothalamic feeding circuits. Mol Metab 5:198-209
Madra, M; Zeltser, L M (2016) BDNF-Val66Met variant and adolescent stress interact to promote susceptibility to anorexic behavior in mice. Transl Psychiatry 6:e776
Kim-Muller, Ja Young; Fan, Jason; Kim, Young Jung R et al. (2016) Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic β cells in diabetic mice. Nat Commun 7:12631
Li, Dylan; Zhang, Feng; Zhang, Xuan et al. (2016) Distinct functions of PPARγ isoforms in regulating adipocyte plasticity. Biochem Biophys Res Commun 481:132-138

Showing the most recent 10 out of 192 publications