The MTCC Imaging &Histology Core offers a suite of modern digital imaging services to biomedical nvestigators at the University of North Carolina at Chapel Hill working to develop gene therapy delivery vectors and procedures. Briefly, the Core will support efforts of gene therapy investigators working to optimize the replication and targeting of adeno-associated viral vectors, and lentiviral and paramyxoviral vectors. Additionally, the Core will also support efforts to understand the functional barrier to viral infection offered by mucus and the luminal glycocalyx in the airways of the lung and in the gastrointestinal system, with the aim of improving delivery of viral vectors through the mucus/mucin barriers of these organs. The support offered by the Core falls into three categories. First, the Core offers small animal and macro imaging services to UNC gene therapy facilities, in the form of cryo-cooled CCD-luminometry and conventional CCD cameras for gene expression studies, gamma-camera scintigraphy for lung clearance studies, and CCD camera-equipped fluorescent stereo dissection microscopy for varied purposes. Second, the Core offers a full range of modern digital imaging microscopy services, with a suite of conventional wide field and laser scanning confocal microscopes to gene therapy investigators. Third, investigators also have available full service histology and electron microscopy facilities. With all these services, investigators also receive consultation with technical problems and assistance with problems in image quantitation and data extraction. The services offered are fully consistent with the importance of modern digital image technology to molecular medicine and the development of effective gene therapies.

Public Health Relevance

Histology and digital imaging microscopy enable the gene therapy investigator to determine the targeting specificity and effectiveness of their experimental therapies. Hence, the Imaging &Histology Core of the MTCC is absolutely essential to the mission of providing critical services to this important area of biomedical research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK065988-10
Application #
8448757
Study Section
Special Emphasis Panel (ZDK1-GRB-1)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
10
Fiscal Year
2013
Total Cost
$150,433
Indirect Cost
$48,788
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Duncan, Gregg A; Kim, Namho; Colon-Cortes, Yanerys et al. (2018) An Adeno-Associated Viral Vector Capable of Penetrating the Mucus Barrier to Inhaled Gene Therapy. Mol Ther Methods Clin Dev 9:296-304
Gentzsch, Martina; Mall, Marcus A (2018) Ion Channel Modulators in Cystic Fibrosis. Chest 154:383-393
Terryah, Shawn T; Fellner, Robert C; Ahmad, Saira et al. (2018) Evaluation of a SPLUNC1-derived peptide for the treatment of cystic fibrosis lung disease. Am J Physiol Lung Cell Mol Physiol 314:L192-L205
Gillen, Austin E; Yang, Rui; Cotton, Calvin U et al. (2018) Molecular characterization of gene regulatory networks in primary human tracheal and bronchial epithelial cells. J Cyst Fibros 17:444-453
Muhlebach, Marianne S; Zorn, Bryan T; Esther, Charles R et al. (2018) Initial acquisition and succession of the cystic fibrosis lung microbiome is associated with disease progression in infants and preschool children. PLoS Pathog 14:e1006798
Cholon, Deborah M; Gentzsch, Martina (2018) Recent progress in translational cystic fibrosis research using precision medicine strategies. J Cyst Fibros 17:S52-S60
Porrello, Alessandro; Leslie, Patrick L; Harrison, Emily B et al. (2018) Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking. Nat Commun 9:1988
Trimble, Aaron T; Whitney Brown, A; Laube, Beth L et al. (2018) Hypertonic saline has a prolonged effect on mucociliary clearance in adults with cystic fibrosis. J Cyst Fibros 17:650-656
Panganiban, Ronald A; Sun, Maoyun; Dahlin, Amber et al. (2018) A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis. J Allergy Clin Immunol 142:1469-1478.e2
Muhlebach, Marianne S; Hatch, Joseph E; Einarsson, Gisli G et al. (2018) Anaerobic bacteria cultured from cystic fibrosis airways correlate to milder disease: a multisite study. Eur Respir J 52:

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