; Collaborative interactions between Investigators in the Section of Nephrology and the Department of Cellular and Molecular Physiology at Yale have historically provided much of the experimental evidence that underlies our current understanding of normal kidney function at the cellular, tubular and whole organ level. And yet, at a time when the development of increasingly sophisticated cell and molecular biology techniques has afforded scientists the ability to manipulate the genes and proteins that control these physiologic, processes, many of the investigators involved in these studies have not acquired the technical skills necessary to identify the mechanism(s) that underlie the phenotype that they uncover. The mission of the Yale O'Brien Kidney Center Renal Physiology and Phenotyping Core (Core A) is to utilize our unique expertise in the rigorous study and understanding of renal physiology to provide highly specialized phenotypic analysis of rodents at the systemic, whole kidney and/or individual nephron segment levels. By providing expertise and training in techniques such as tubule micropuncture, in vitro and in vivo microperfusion, determination of glomerular filtration rates and renal perfusion, and continuous blood pressure measurements. Core A is designed to allow investigators to accurately define the site, mechanism and impact of genetic and/or pharmacologic manipulations on cellular, organ, and whole animal physiology. To achieve this, the Yale O'Brien Kidney Center Renal Physiology and Phenotyping Core takes advantage of 2 major strengths: 1) the specialized equipment needed to provide phenotyping .services at the systemic, whole kidney, and individual nephron segment levels, and 2) experienced and skilled personnel capable of using that equipment to generate reliable and reproducible data that are required for thorough, accurate phenotypic analysis. During the previous funding period, this Core was heavily utilized by Investigators with 19 distinct services provided to more than 40 Investigators supporting studies in 32 manuscripts.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-6 (M2))
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Yale University
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Khan, Usman A; Coca, Steven G; Hong, Kwangik et al. (2014) Blood transfusions are associated with urinary biomarkers of kidney injury in cardiac surgery. J Thorac Cardiovasc Surg 148:726-32
Parikh, Chirag R; Thiessen-Philbrook, Heather (2014) Key concepts and limitations of statistical methods for evaluating biomarkers of kidney disease. J Am Soc Nephrol 25:1621-9
Hornik, Christoph P; Krawczeski, Catherine D; Zappitelli, Michael et al. (2014) Serum brain natriuretic peptide and risk of acute kidney injury after cardiac operations in children. Ann Thorac Surg 97:2142-7
Molnar, Amber O; Parikh, Chirag R; Coca, Steven G et al. (2014) Association between preoperative statin use and acute kidney injury biomarkers in cardiac surgical procedures. Ann Thorac Surg 97:2081-7
Hall, I E; Bhangoo, R S; Reese, P P et al. (2014) Glutathione S-transferase iso-enzymes in perfusate from pumped kidneys are associated with delayed graft function. Am J Transplant 14:886-96
Stark, Romana; Guebre-Egziabher, Fitsum; Zhao, Xiaojian et al. (2014) A role for mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) in the regulation of hepatic gluconeogenesis. J Biol Chem 289:7257-63
Wang, Ling; Velazquez, Heino; Moeckel, Gilbert et al. (2014) Renalase prevents AKI independent of amine oxidase activity. J Am Soc Nephrol 25:1226-35
Fretz, Jackie A; Nelson, Tracy; Velazquez, Heino et al. (2014) Early B-cell factor 1 is an essential transcription factor for postnatal glomerular maturation. Kidney Int 85:1091-102
Belcher, Justin M; Garcia-Tsao, Guadalupe; Sanyal, Arun J et al. (2014) Urinary biomarkers and progression of AKI in patients with cirrhosis. Clin J Am Soc Nephrol 9:1857-67
Keramati, Ali R; Fathzadeh, Mohsen; Go, Gwang-Woong et al. (2014) A form of the metabolic syndrome associated with mutations in DYRK1B. N Engl J Med 370:1909-19

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