Advances in optical imaging technologies have equipped researchers with extremely powerful tools to uniquely address clinically and biologically important questions that can only be accomplished in whole organ studies. Researchers equipped with these unique and ever improving tools can utilize optical microscopy and digital image analysis to study subcellular events within cells, cell-cell Interactions and integrative organ physiology, biochemistry, molecular and cellular biology. This has greatly enhanced the understanding of physiologic and disease processes, developmental biology, and has hastened and improved the reliability and interpretation of preclinical data. Multi-photon microscopy offers the investigator a minimally invasive high resolution technique with Increased depth of penetration and markedly reduced phototoxicity for visualization of cell-cell and intracellular events intravitally. A tremendous strength of the present O'Brien Center is our ability to customize our approach to individual user's needs. The Intravital Multi-photon Microscopy Core provides an extensive range of services including access to the necessary ICBM facilities and expertise in all aspects of intravital multiphoton imaging from utilization and training at the hands-on level to the theoretical aspects of microscopy, cell and molecular biology and kidney systems physiology.

Public Health Relevance

The Indiana O'Brien Center is founded upon the mission of developing and implementing methods of microscopy that provide unique and powerful insights into renal function and dysfunction. The Intravital Multiphoton Microscopy Core will play a critical role in advancing understanding of the pathophysiology of in vivo disease processes, and the mechanisms of therapeutic approaches.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK079312-06
Application #
8433633
Study Section
Special Emphasis Panel (ZDK1-GRB-6 (M1))
Project Start
Project End
Budget Start
2012-08-20
Budget End
2013-06-30
Support Year
6
Fiscal Year
2012
Total Cost
$274,590
Indirect Cost
$98,571
Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
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Winfree, Seth; Ferkowicz, Michael J; Dagher, Pierre C et al. (2017) Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and translational implications. Transl Res 189:1-12

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