Advances in optical imaging technologies have equipped researchers with extremely powerful tools to uniquely address clinically and biologically important questions that can only be accomplished in whole organ studies. Researchers equipped with these unique and ever improving tools can utilize optical microscopy and digital image analysis to study subcellular events within cells, cell-cell Interactions and integrative organ physiology, biochemistry, molecular and cellular biology. This has greatly enhanced the understanding of physiologic and disease processes, developmental biology, and has hastened and improved the reliability and interpretation of preclinical data. Multi-photon microscopy offers the investigator a minimally invasive high resolution technique with Increased depth of penetration and markedly reduced phototoxicity for visualization of cell-cell and intracellular events intravitally. A tremendous strength of the present O'Brien Center is our ability to customize our approach to individual user's needs. The Intravital Multi-photon Microscopy Core provides an extensive range of services including access to the necessary ICBM facilities and expertise in all aspects of intravital multiphoton imaging from utilization and training at the hands-on level to the theoretical aspects of microscopy, cell and molecular biology and kidney systems physiology.

Public Health Relevance

The Indiana O'Brien Center is founded upon the mission of developing and implementing methods of microscopy that provide unique and powerful insights into renal function and dysfunction. The Intravital Multiphoton Microscopy Core will play a critical role in advancing understanding of the pathophysiology of in vivo disease processes, and the mechanisms of therapeutic approaches.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK079312-07
Application #
8534770
Study Section
Special Emphasis Panel (ZDK1-GRB-6)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
7
Fiscal Year
2013
Total Cost
$274,590
Indirect Cost
$98,571
Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
Atkinson, Simon J (2016) A wandering path toward prevention for acute kidney injury. J Clin Invest 126:1640-2
Chen, Z; Wan, X; Hou, Q et al. (2016) GADD45B mediates podocyte injury in zebrafish by activating the ROS-GADD45B-p38 pathway. Cell Death Dis 7:e2068
Wagner, Mark C; Campos-Bilderback, Silvia B; Chowdhury, Mahboob et al. (2016) Proximal Tubules Have the Capacity to Regulate Uptake of Albumin. J Am Soc Nephrol 27:482-94
de Almeida, Rita M C; Clendenon, Sherry G; Richards, William G et al. (2016) Transcriptome analysis reveals manifold mechanisms of cyst development in ADPKD. Hum Genomics 10:37
Molitoris, Bruce A; Reilly, Erinn S (2016) Quantifying Glomerular Filtration Rates in Acute Kidney Injury: A Requirement for Translational Success. Semin Nephrol 36:31-41
Hato, Takashi; Sandoval, Ruben; Dagher, Pierre C (2015) The caspase 3 sensor Phiphilux G2D2 is activated non-specifically in S1 renal proximal tubules. Intravital 4:
Tao, Wen; Rubart, Michael; Ryan, Jennifer et al. (2015) A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy. Am J Physiol Cell Physiol 309:C724-35
Hato, Takashi; Dagher, Pierre C (2015) How the Innate Immune System Senses Trouble and Causes Trouble. Clin J Am Soc Nephrol 10:1459-69
El-Achkar, Tarek M; Dagher, Pierre C (2015) Tubular cross talk in acute kidney injury: a story of sense and sensibility. Am J Physiol Renal Physiol 308:F1317-23
Hato, Takashi; Winfree, Seth; Kalakeche, Rabih et al. (2015) The macrophage mediates the renoprotective effects of endotoxin preconditioning. J Am Soc Nephrol 26:1347-62

Showing the most recent 10 out of 68 publications