The cloning and engineering of genetically encoded fluorescent proteins has allowed the development of many unique tools for studies in cell biology, medicine, and physiology. When combined with intravital microscopy, these probes have the potential to report events from inside living cells in the intact organism. However, the utility of the fluorescent protein biosensors for intravital microscopy has been limited by the poorly characterized behaviors of fluorescent proteins under two-photon excitation. The goal of the Multiphoton Biosensor Development core is to develop and rigorously validate a set of biosensor probes optimized for intravital multiphoton microscopy studies of renal function. Modular probes developed by the core will be incorporated into a toolkit of probes and protocols that will be provided to renal investigators. These probes will provide renal researchers with powerful new tools that will enable them to conduct novel intravital microscopy studies addressing fundamental issues of renal physiology, cell biology and pathophysiology.
The Indiana O'Brien Center is founded upon the mission of developing and implementing methods of microscopy that provide unique and powerful insights into renal function and dysfunction. The Multi-photon Biosensor Development Core will play a critical role in this mission, developing novel biosensors that will support unique microscopy studies.
|Day, Richard N (2014) Measuring protein interactions using Forster resonance energy transfer and fluorescence lifetime imaging microscopy. Methods 66:200-7|
|Hall, Andrew M; Molitoris, Bruce A (2014) Dynamic multiphoton microscopy: focusing light on acute kidney injury. Physiology (Bethesda) 29:334-42|
|Kapitsinou, Pinelopi P; Sano, Hideto; Michael, Mark et al. (2014) Endothelial HIF-2 mediates protection and recovery from ischemic kidney injury. J Clin Invest 124:2396-409|
|Dickson, Landon E; Wagner, Mark C; Sandoval, Ruben M et al. (2014) The proximal tubule and albuminuria: really! J Am Soc Nephrol 25:443-53|
|Sandoval, Ruben M; Wang, Exing; Molitoris, Bruce A (2014) Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy. Intravital 2:|
|Sandoval, Ruben M; Molitoris, Bruce A (2013) Quantifying glomerular permeability of fluorescent macromolecules using 2-photon microscopy in Munich Wistar rats. J Vis Exp :|
|Shaner, Nathan C; Lambert, Gerard G; Chammas, Andrew et al. (2013) A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods 10:407-9|
|Werner, Michael E; Ward, Heather H; Phillips, Carrie L et al. (2013) Inversin modulates the cortical actin network during mitosis. Am J Physiol Cell Physiol 305:C36-47|
|Blaeser, Anthony; Keramaris, Elizabeth; Chan, Yiumo M et al. (2013) Mouse models of fukutin-related protein mutations show a wide range of disease phenotypes. Hum Genet 132:923-34|
|Hato, Takashi; El-Achkar, Tarek M; Dagher, Pierre C (2013) Sisters in arms: myeloid and tubular epithelial cells shape renal innate immunity. Am J Physiol Renal Physiol 304:F1243-51|
Showing the most recent 10 out of 34 publications