The Digital Image Analysis core will complement the Intravital Multi-photon Microscopy Core by providing digital image processing services for O'Brien investigators to support data exploration and quantification. The Core will provide analysis services, will train investigators in the use of image analysis software and will develop new tools to enhance and extend the visualization and quantification of deep tissue microscopy images. In particular the Digital Image Analysis core will develop and distribute user-friendly software that will provide effective, efficient tools for quantifying 2-dimensional and 3-dimensional fluorescence microscopy images. These services will be made available remotely through the dissemination of free, flexible image analysis software and an interactive televisualization system. The Digital Image Analysis Core will provide researchers with powerful new tools enabling them to conduct novel studies addressing fundamental issues of renal physiology, cell biology and pathophysiology.

Public Health Relevance

The Indiana OBrien Center Is founded upon the mission of developing and implementing methods of microscopy that provide unique and powerful insights into renal function and dysfunction. The Digital Image Analysis Core plays a critical role in this mission, developing critical tools to explore and quantify microscopy studies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK079312-08
Application #
8726962
Study Section
Special Emphasis Panel (ZDK1-GRB-6)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
8
Fiscal Year
2014
Total Cost
$197,763
Indirect Cost
$46,846
Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
Day, Richard N (2014) Measuring protein interactions using Forster resonance energy transfer and fluorescence lifetime imaging microscopy. Methods 66:200-7
Hall, Andrew M; Molitoris, Bruce A (2014) Dynamic multiphoton microscopy: focusing light on acute kidney injury. Physiology (Bethesda) 29:334-42
Kapitsinou, Pinelopi P; Sano, Hideto; Michael, Mark et al. (2014) Endothelial HIF-2 mediates protection and recovery from ischemic kidney injury. J Clin Invest 124:2396-409
Dickson, Landon E; Wagner, Mark C; Sandoval, Ruben M et al. (2014) The proximal tubule and albuminuria: really! J Am Soc Nephrol 25:443-53
Sandoval, Ruben M; Wang, Exing; Molitoris, Bruce A (2014) Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy. Intravital 2:
Sandoval, Ruben M; Molitoris, Bruce A (2013) Quantifying glomerular permeability of fluorescent macromolecules using 2-photon microscopy in Munich Wistar rats. J Vis Exp :
Shaner, Nathan C; Lambert, Gerard G; Chammas, Andrew et al. (2013) A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods 10:407-9
Werner, Michael E; Ward, Heather H; Phillips, Carrie L et al. (2013) Inversin modulates the cortical actin network during mitosis. Am J Physiol Cell Physiol 305:C36-47
Blaeser, Anthony; Keramaris, Elizabeth; Chan, Yiumo M et al. (2013) Mouse models of fukutin-related protein mutations show a wide range of disease phenotypes. Hum Genet 132:923-34
Hato, Takashi; El-Achkar, Tarek M; Dagher, Pierre C (2013) Sisters in arms: myeloid and tubular epithelial cells shape renal innate immunity. Am J Physiol Renal Physiol 304:F1243-51

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