Genetically modified mice are important tools for studying kidney physiology and diseases. However, expertise and equipment for physiological analysis of kidney function in mice are not readily available to many investigators in the renal research community. Furthermore, production of mutant mice has allowed many investigators outside the renal community to uncover unexpected renal phenotypes and enter into renal research. The objective of the Physiology Core is to assist center investigators with a wide range of physiological techniques to study kidney physiology and diseases at the cellular, organ, and whole animal level. To achieve these objectives, the Core will provide the following specific services: (1) Whole animal clearance studies;(2) Measurements of serum and urine electrolytes and chemistry;(3) Measurement of blood pressure;(4) Measurement of electrolytes in nl volume using ion-selective electrode (5) Isolation of individual tubules for real-time PCR and immuno-fluorescent staining;(6) In vitro microperfusion of individually isolated tubules;(7) Electrophysiological studies. Procedures will be in place to evaluate efficiency and to maintain appropriate quality control of the services that are provided. The Core will also provide education and training to the staffs of center investigators. The core directors have a track record of providing assistance and training to visiting scientists locally and nationally. The Core director. Dr. Chou- Long Huang, received his PhD in Physiology from UCSF working with Dr. Floyd Rector on renal physiology and completed postdoctoral training with Dr. Lily Jan at UCSF working on K+ channels. He is well recognized for his studies of renal ion channels. His laboratory actively employs electrophysiological and other physiological approaches and mouse models. The co-director. Dr. Michel Baum, also trained in Dr. Floyd Rector's lab in UCSF. Throughout his independent research career, he has studied renal ion transport using in vitro microperfusion and microanalytic and imaging techniques on individually isolated tubules. The Physiology Core will be housed in the laboratories of the core directors in the Departments of Internal Medicine and Pediatrics at UTSW.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK079328-08
Application #
8721932
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
8
Fiscal Year
2014
Total Cost
Indirect Cost
City
Dallas
State
TX
Country
United States
Zip Code
75390
Baum, Michel (2016) Luminal angiotensin II stimulates rat medullary thick ascending limb chloride transport in the presence of basolateral norepinephrine. Am J Physiol Renal Physiol 310:F294-9
Tanriover, Bekir; MacConmara, Malcolm P; Parekh, Justin et al. (2016) SIMULTANEOUS LIVER KIDNEY TRANSPLANTATION IN LIVER TRANSPLANT CANDIDATES WITH RENAL DYSFUNCTION: IMPORTANCE OF CREATININE LEVELS, DIALYSIS, AND ORGAN QUALITY IN SURVIVAL. KI Rep 1:221-229
Zhang, Jiandong; Rudemiller, Nathan P; Patel, Mehul B et al. (2016) Competing Actions of Type 1 Angiotensin II Receptors Expressed on T Lymphocytes and Kidney Epithelium during Cisplatin-Induced AKI. J Am Soc Nephrol 27:2257-64
Srivastava, Anand; Adams-Huet, Beverley; Vega, Gloria L et al. (2016) Effect of losartan and spironolactone on triglyceride-rich lipoproteins in diabetic nephropathy. J Investig Med 64:1102-8
Baum, Michel (2016) Neonatal nephrology. Curr Opin Pediatr 28:170-2
Yang, Zhufeng; Zimmerman, Susan E; Tsunezumi, Jun et al. (2016) Role of CD34 family members in lumen formation in the developing kidney. Dev Biol 418:66-74
Westover, Arthur N; Nakonezny, Paul A; Barlow, Carolyn E et al. (2016) Heart Rate Recovery and Systolic Blood Pressure Recovery After Maximal Exercise in Prevalent Users of Stimulant Medications. J Clin Psychopharmacol 36:295-7
Jain, Nishank; Li, Xilong; Adams-Huet, Beverley et al. (2016) Differences in Whole Blood Platelet Aggregation at Baseline and in Response to Aspirin and Aspirin Plus Clopidogrel in Patients With Versus Without Chronic Kidney Disease. Am J Cardiol 117:656-63
Ravikumar, Priya; Menon, Jyothi U; Punnakitikashem, Primana et al. (2016) Nanoparticle facilitated inhalational delivery of erythropoietin receptor cDNA protects against hyperoxic lung injury. Nanomedicine 12:811-21
Vongpatanasin, Wanpen; Peri-Okonny, Poghni; Velasco, Alejandro et al. (2016) Effects of Potassium Magnesium Citrate Supplementation on 24-Hour Ambulatory Blood Pressure and Oxidative Stress Marker in Prehypertensive and Hypertensive Subjects. Am J Cardiol 118:849-53

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