The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. Core C has been successful in supporting the kidney research community. Since the inception of Core C, more than 8,500 assays have been performed for 48 principal investigators involving 56 projects. Of the 48 investigators, 44 were non-core personnel. The number of investigators using Core C each year is increasing, as is the annual publication rate. Core C has also supported the research efforts of 7 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 23 peer-reviewed publications. The Core will participate in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature from 2013-2017 will be an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics). Finally, the Bioanalytical Core, in concert with the O'Brien Center leadership, will identify new bioanalytical needs of investigators and implement them for the Center.

Public Health Relevance

Acute kidney injury is associated with severe consequences including death, prolonged hospitalization and can lead to chronic kidney disease. Core C of this Center provides investigators key resources required to enable a better understanding of the underlying mechanisms and determine best approaches to diagnose and manage this disease.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-6 (M2))
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University of Alabama Birmingham
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Rao, Sangeetha; Walters, Kelly B; Wilson, Landon et al. (2016) Early lipid changes in acute kidney injury using SWATH lipidomics coupled with MALDI tissue imaging. Am J Physiol Renal Physiol 310:F1136-47
Jackson, Jennifer L; Judd, Suzanne E; Panwar, Bhupesh et al. (2016) Associations of 25-hydroxyvitamin D with markers of inflammation, insulin resistance and obesity in black and white community-dwelling adults. J Clin Transl Endocrinol 5:21-25
Awdishu, Linda; Joy, Melanie S (2016) Role of Pharmacogenomics in Kidney Disease and Injury. Adv Chronic Kidney Dis 23:106-19
Hull, Travis D; Boddu, Ravindra; Guo, Lingling et al. (2016) Heme oxygenase-1 regulates mitochondrial quality control in the heart. JCI Insight 1:e85817
Smith, M Ryan; Vayalil, Praveen K; Zhou, Fen et al. (2016) Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels. Redox Biol 8:136-48
Williams, Jennifer; Holmes, Ross P; Assimos, Dean G et al. (2016) Monocyte Mitochondrial Function in Calcium Oxalate Stone Formers. Urology 93:224.e1-6
Vallon, Volker (2016) Tubular Transport in Acute Kidney Injury: Relevance for Diagnosis, Prognosis and Intervention. Nephron 134:160-166
Bolisetty, Subhashini; Traylor, Amie; Joseph, Reny et al. (2016) Proximal tubule-targeted heme oxygenase-1 in cisplatin-induced acute kidney injury. Am J Physiol Renal Physiol 310:F385-94
Weir, Matthew R; Blantz, Roland C (2016) Noteworthy observations in hypertension from 2015. Curr Opin Nephrol Hypertens 25:1-2
Leaf, David E; Body, Simon C; Muehlschlegel, Jochen D et al. (2016) Length Polymorphisms in Heme Oxygenase-1 and AKI after Cardiac Surgery. J Am Soc Nephrol 27:3291-3297

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