During the past funding cycle, the establishment of the services provided by the Cell Biology Core has fostered a large variety of research related to the Core Objectives. Cell Biological Studies related to the DRTC have expanded within the research community as evidenced by publications as well as grant applications from investigatoVs who traditionally do not work in the area of diabetes mellitus and metabolism. The adipose tissue bank and adipose biology expertise and service of the DRTC was transferred to operate under the auspices of the newly funded Mid-Atlantic nutrition and obesity research center (NORC) at the University of Maryland. This ensured a strengthening and continuity of adipose tissue related services to the DRTC community while freeing resources, which were used for innovation and expansion of services through the Cell Biology Core of the Baltimore DRTC. The Cell Biology Core started a local repository of transgenic mouse models of tissue/cell specific transgenic CRE -deleter ordoxycylcine regulatable mice. These well-characterized mice, in the C57BI/6 background, have become a valuable resource for the research community. Responding to investigators'needs as well as to pertinent questions related to metabolism and diabetes mellitus, the Cell Biology has expanded its services and expertise in the area of cellular bioenergetics and mitochondrial biology. This new addition will provide expertise and services to image and quantify mitochondrial content and mitochondrial shape and constellation, to assess mitochondrial dynamics (fusion and division), provide expertise in quantification of mitochondrial gene and protein expression (e.g. uncoupling proteins, complexes of the electron transfer chain) and cellular mitochondrial DNA content. In addition DRC users will gain access to unique genetic mouse models (e.g. floxed Drp1, floxeci Opal mice), which allow conditional tissue specific ablation of proteins involved in mitochondrial fusion and division and in mediating apoptosis in response to oxidative cellular stress.

Public Health Relevance

The Cell Biology Core provides efficient and economical access to expertise and services related to tissues Important, in the regulation of energy metabolism and in the pathogenesis of diabetes mellitus and its complications.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1-GRB-S (O2))
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Johns Hopkins University
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Min, Jungwon; Li, Zhu; Liu, Xia et al. (2014) The association between early menarche and offspring's obesity risk in early childhood was modified by gestational weight gain. Obesity (Silver Spring) 22:19-23
Shin, Mi-Kyung; Han, Woobum; Bevans-Fonti, Shannon et al. (2014) The effect of adrenal medullectomy on metabolic responses to chronic intermittent hypoxia. Respir Physiol Neurobiol 203:60-7
Ivey, Frederick M; Ryan, Alice S (2014) Resistive training improves insulin sensitivity after stroke. J Stroke Cerebrovasc Dis 23:225-9
Kuang, Zheng; Cai, Ling; Zhang, Xuekui et al. (2014) High-temporal-resolution view of transcription and chromatin states across distinct metabolic states in budding yeast. Nat Struct Mol Biol 21:854-63
Chatterjee, Ranee; Brancati, Frederick L; Shafi, Tariq et al. (2014) Non-traditional risk factors are important contributors to the racial disparity in diabetes risk: the atherosclerosis risk in communities study. J Gen Intern Med 29:290-7
Gabelli, Sandra B; Echeverria, Ignacia; Alexander, Megan et al. (2014) Activation of PI3K? by physiological effectors and by oncogenic mutations: structural and dynamic effects. Biophys Rev 6:89-95
Zhang, Tao; Baldie, Gerard; Periz, Goran et al. (2014) RNA-processing protein TDP-43 regulates FOXO-dependent protein quality control in stress response. PLoS Genet 10:e1004693
Albert, Jessica S; Yerges-Armstrong, Laura M; Horenstein, Richard B et al. (2014) Null mutation in hormone-sensitive lipase gene and risk of type 2 diabetes. N Engl J Med 370:2307-15
Xie, Hui; Cui, Zhuang; Wang, Long et al. (2014) PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis. Nat Med 20:1270-8
Cortassa, Sonia; O'Rourke, Brian; Aon, Miguel A (2014) Redox-optimized ROS balance and the relationship between mitochondrial respiration and ROS. Biochim Biophys Acta 1837:287-95

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