The Transgenic and ES Cell Core of the JHU-UMD DRC will enable Members at Johns Hopkins University and the University of Maryland to efficiently generate transgenic and knock-out/in mice. Fredric E. Wondisford, M.D. will serve as Director, and Sally Radovick, M.D. will serve as Co-Director. Both are highly qualified to direct this core and their direct involvement in diabetes and diabetes-related research should further enhance transgenic/knock-out services to JHU-UMD investigators. The Center will utilize the existing Transgenic Core at Johns Hopkins University School of Medicine with the addition of dedicated technical support to expedite generation of mouse models for DRC Members and at a lesser cost. The DRC will continue to generate targeted ES cells using highly reliable and efficient protocols. The intellectual aspects associated with the generation of transgenic and knock-out/knock-in constructs and the reagents necessary for ES cells generation have proved to important for many investigators. By concentrating experience and expertise in this Core facility, the Core will markedly accelerate the progress of discovery for Center investigators. Transgenic services will be located in JHU space that is already allocated to the existing facility, and this facility has adequate equipment. ES cell services will be located in the connected CMSC building, adjacent to Dr. Wondisford's laboratories, in 500 sq. ft. of space. Drs. Wondisford and Radovick will assist in the design of targeting constructs. The Core will be managed by Dr. Sidhaye, who will oversee the introduction of constructs into ES cells, identifying ES clones that have undergone homologous recombination along with the respective investigator's laboratory and preparation of cells for injection into blastocysts. Facilitating transport of genetically altered mice from JHU to UMD has been part of the Core's function. Alternatively, budgeted into the Core'are short-term housing costs at JHU for mice made for UMD Members, if their study protocol requires imaging or metabolic studies.

Public Health Relevance

The Transgenic and ES Cell Core of the JHU-UMD DRC enables Members at Johns Hopkins University and the University of Maryland to efficiently generate transgenic and knock-out/in mice for use in the study of diabetes mechanisms. This proposal seeks funding to continue this core acitivty.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK079637-06
Application #
8443956
Study Section
Special Emphasis Panel (ZDK1-GRB-S (O2))
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
6
Fiscal Year
2013
Total Cost
$183,319
Indirect Cost
$70,159
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Fesseha, Betiel K; Abularrage, Christopher J; Hines, Kathryn F et al. (2018) Association of Hemoglobin A1c and Wound Healing in Diabetic Foot Ulcers. Diabetes Care 41:1478-1485
Bilal, Usama; Hill-Briggs, Felicia; Sánchez-Perruca, Luis et al. (2018) Association of neighbourhood socioeconomic status and diabetes burden using electronic health records in Madrid (Spain): the HeartHealthyHoods study. BMJ Open 8:e021143
Kushwaha, Priyanka; Wolfgang, Michael J; Riddle, Ryan C (2018) Fatty acid metabolism by the osteoblast. Bone 115:8-14
Kim, Soohyun P; Frey, Julie L; Li, Zhu et al. (2017) Sclerostin influences body composition by regulating catabolic and anabolic metabolism in adipocytes. Proc Natl Acad Sci U S A 114:E11238-E11247
Kim, Soohyun P; Frey, Julie L; Li, Zhu et al. (2017) Lack of Lrp5 Signaling in Osteoblasts Sensitizes Male Mice to Diet-Induced Disturbances in Glucose Metabolism. Endocrinology 158:3805-3816
Winters, Alexandra; Ramos-Molina, Bruno; Jarvela, Timothy S et al. (2017) Functional analysis of PCSK2 coding variants: A founder effect in the Old Order Amish population. Diabetes Res Clin Pract 131:82-90
Riddle, Ryan C; Clemens, Thomas L (2017) Bone Cell Bioenergetics and Skeletal Energy Homeostasis. Physiol Rev 97:667-698
Saloustros, Emmanouil; Salpea, Paraskevi; Starost, Matthew et al. (2017) Prkar1a gene knockout in the pancreas leads to neuroendocrine tumorigenesis. Endocr Relat Cancer 24:31-40
Hassoon, Ahmed; Bydon, Mohamad; Kerezoudis, Panagiotis et al. (2017) Chronic low-back pain in adult with diabetes: NHANES 2009-2010. J Diabetes Complications 31:38-42
Kim, Soohyun P; Li, Zhu; Zoch, Meredith L et al. (2017) Fatty acid oxidation by the osteoblast is required for normal bone acquisition in a sex- and diet-dependent manner. JCI Insight 2:

Showing the most recent 10 out of 755 publications