The Optical Morphology Core will play an essential role in supporting the overall goals of the Center.
The Specific Aims of this core are three-fold. First, to provide reliable, accessible, and state-of-the-art microscopic technology to all Center members that will facilitate their study of Gl cellular signaling cascades. Second, to educate and train Center members in the use of both basic and sophisticated cellular imaging methods. Emphasis will be placed on providing technical instruction as well as educating faculty about how such approaches can expand the scope and breadth of their scientific programs. Third, development and application of state-of-the-art optical imaging technologies to Gl tissues/cells including;high resolution, real-time computer/video imaging of live cells;confocal microscopy coupled with computer-based 3-D image reconstruction;Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein-protein interactions;Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover;expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids;and the development and application of specific photoactivatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK084567-04
Application #
8381869
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
4
Fiscal Year
2012
Total Cost
$232,305
Indirect Cost
$78,562
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
006471700
City
Rochester
State
MN
Country
United States
Zip Code
55905
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Mauer, Amy S; Hirsova, Petra; Maiers, Jessica L et al. (2017) Inhibition of sphingosine 1-phosphate signaling ameliorates murine nonalcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 312:G300-G313
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