The Optical Morphology Core will play an essential role in supporting the overall goals of the Center.
The Specific Aims of this core are three-fold. First, to provide reliable, accessible, and state-of-the-art microscopic technology to all Center members that will facilitate their study of Gl cellular signaling cascades. Second, to educate and train Center members in the use of both basic and sophisticated cellular imaging methods. Emphasis will be placed on providing technical instruction as well as educating faculty about how such approaches can expand the scope and breadth of their scientific programs. Third, development and application of state-of-the-art optical imaging technologies to Gl tissues/cells including;high resolution, real-time computer/video imaging of live cells;confocal microscopy coupled with computer-based 3-D image reconstruction;Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein-protein interactions;Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover;expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids;and the development and application of specific photoactivatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells.
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|Tomita, Kyoko; Kohli, Rohit; MacLaurin, Brittany L et al. (2017) Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis. JCI Insight 2:|
|Rizvi, Sumera; Gores, Gregory J (2017) Emerging molecular therapeutic targets for cholangiocarcinoma. J Hepatol 67:632-644|
|Eisenman, S T; Gibbons, S J; Verhulst, P-J et al. (2017) Tumor necrosis factor alpha derived from classically activated ""M1"" macrophages reduces interstitial cell of Cajal numbers. Neurogastroenterol Motil 29:|
|Strege, Peter R; Gibbons, Simon J; Mazzone, Amelia et al. (2017) EAVK segment ""c"" sequence confers Ca2+-dependent changes to the kinetics of full-length human Ano1. Am J Physiol Gastrointest Liver Physiol 312:G572-G579|
|Schott, Micah B; Rasineni, Karuna; Weller, Shaun G et al. (2017) ?-Adrenergic induction of lipolysis in hepatocytes is inhibited by ethanol exposure. J Biol Chem 292:11815-11828|
|Alcaino, C; Farrugia, G; Beyder, A (2017) Mechanosensitive Piezo Channels in the Gastrointestinal Tract. Curr Top Membr 79:219-244|
|Sarmento, Olga F; Svingen, Phyllis A; Xiong, Yuning et al. (2017) The Role of the Histone Methyltransferase Enhancer of Zeste Homolog 2 (EZH2) in the Pathobiological Mechanisms Underlying Inflammatory Bowel Disease (IBD). J Biol Chem 292:706-722|
|Mauer, Amy S; Hirsova, Petra; Maiers, Jessica L et al. (2017) Inhibition of sphingosine 1-phosphate signaling ameliorates murine nonalcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 312:G300-G313|
|Khanna, Sahil; Vazquez-Baeza, Yoshiki; González, Antonio et al. (2017) Changes in microbial ecology after fecal microbiota transplantation for recurrent C. difficile infection affected by underlying inflammatory bowel disease. Microbiome 5:55|
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