The objective of the C-SiG Optical Microscopy Core, is to be a state-of-the-art, user-friendly service that connects investigators with the many optical technologies and applications at a reasonable cost. Under the direction of Dr. Mark McNiven, a well-established cell biologist, the Core integrates existing resources from the Mayo Microscopy and Cell Analysis Core and from individual investigators in the Division of Gastroenterology and Hepatology (GIH) as well as providing additional new technologies not previously available.
The Specific Aims of this core are three-fold. First, to provide reliable, accessible, state-of-the-art microscopic technology to all C-SiG members that will facilitate their study of Gl cellular signaling cascades. Second, to educate and train C-SiG members;in the use of both basic and sophisticated cellular imaging methods. Emphasis is placed on providing technical instruction as well as educating faculty on how such approaches can expand the scope and breadth of their scientific programs. Third, to develop and apply state-of-the-art optical imaging technologies, including fluorescent probes and biosensors, to study Gl tissues and/or cells. The most popular Core service is access to the well-maintained C-SiG Confocal Microscopes. The Core also provides instruction, technical advice, data interpretation, and development of novel, innovative optical approaches to the study of signaling pathways in Gl cells and tissues. These services cover a wide range of topics including: real-time computer/video imaging of live cells;confocal microscopy coupled with computer-based 3-D image reconstruction;Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein-protein interactions;Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover;Fluorescence Loss in Photobleaching (FLIP);microinjection of living cells;expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids;the development and application of specific photo-activatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells;Total internal reflection (TIRF) microscopy; multiphoton microscopy, and super-resolution microscopy. Core services have been used by 58% of CSiG members and supported 106 publications.

Public Health Relevance

Gastrointestinal diseases and their complications have a significant effect on public health and health care utilization costs. The C-SiG Optical Microscopy Core supports scientific advancements of C-SiG members that are critically important for furthering understanding of the mechanisms that underlie digestive diseases, which can lead to practical applications for the diagnosis, prevention, monitoring and treatment of human disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK084567-06
Application #
8737722
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905
Ni, Jun; Wangensteen, Kirk J; Nelsen, David et al. (2016) Active recombinant Tol2 transposase for gene transfer and gene discovery applications. Mob DNA 7:6
Landry, Greg M; Hirata, Taku; Anderson, Jacob B et al. (2016) Sulfate and thiosulfate inhibit oxalate transport via a dPrestin (Slc26a6)-dependent mechanism in an insect model of calcium oxalate nephrolithiasis. Am J Physiol Renal Physiol 310:F152-9
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Tomita, Kyoko; Freeman, Brittany L; Bronk, Steven F et al. (2016) CXCL10-Mediates Macrophage, but not Other Innate Immune Cells-Associated Inflammation in Murine Nonalcoholic Steatohepatitis. Sci Rep 6:28786
Kawakami, Hisato; Huang, Shengbing; Pal, Krishnendu et al. (2016) Mutant BRAF Upregulates MCL-1 to Confer Apoptosis Resistance that Is Reversed by MCL-1 Antagonism and Cobimetinib in Colorectal Cancer. Mol Cancer Ther 15:3015-3027
Tabibian, James H; Varghese, Cyril; LaRusso, Nicholas F et al. (2016) The enteric microbiome in hepatobiliary health and disease. Liver Int 36:480-7
Verma, Vikas K; Li, Haiyang; Wang, Ruisi et al. (2016) Alcohol stimulates macrophage activation through caspase-dependent hepatocyte derived release of CD40L containing extracellular vesicles. J Hepatol 64:651-60
Ding, Xiwei; Chaiteerakij, Roongruedee; Moser, Catherine D et al. (2016) Antitumor effect of the novel sphingosine kinase 2 inhibitor ABC294640 is enhanced by inhibition of autophagy and by sorafenib in human cholangiocarcinoma cells. Oncotarget 7:20080-92

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