The Hopkins Digestive Diseases Basic and Translational Research Core Center is a proposed Silvio Conte Core Center which has been designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine. This Core Center has as its theme, """"""""Regulation of Epithelial Function, Especially via Changes in Signal Transduction, Trafficking and Development"""""""". It has a Research Base of 38 full Members and 12 Associate Members. It consists of: 4 scientific Cores and an Administrative Core. Core A, Proteomics Core will help identify proteins that are differentially expressed in GI tract/liver normally or in diseases, interactions among proteins including identification of binding partners, will map cleavage sites and determine post-translational modifications. Core B, Imaging Core will make multiple types of cutting edge imaging and EM (scanning, transmission, immuno) of epithelial cells available to Core Center investigators and provide a Core Manager to help perform the studies. These include confocal and two-photon microscopy with FRAP, FRET and TIRF;use of fluorometers;single cell quantitative imaging;digital camera;access to Laser Capture Microscopy. Core C, Mouse Physiology Core will provide advice in establishing mouse colonies;genotype mouse models of digestive diseases;make available metabolic cages and perform urine, stool, blood collections and analysis. This Core will also perform aortic perfusion of mice as part of intestinal, liver pancreas, kidney procurement and preparation for Northern and Western analysis and histological slide preparation. A mouse pathologist, cytokine multiplex ELISA and a FACS technician are new components of Core C. Core C makes equipment available and provides instruction in its use for Ussing chamber/voltage clamp studies. Core D, Translational Research Enhancement Core provides help in forming a clinical database and specimen collection from patients for DNA, RNA, specimen procurement Administrative Core will provide the communication, financial management and administrative functions of the Core Center, organize the Enrichment Program and administer the Pilot Project and Mini-sabbatical Programs.
This Conte Gl Core Center provides Administrative and Scientific Cores to advance the digestive diseases research of its 50 members Research Base in which the theme studied relates to regulation of epithelial function, especially via changes in signal transduction, trafficking, and development of Gl tissues.
|Housseau, Franck; Wu, Shaoguang; Wick, Elizabeth C et al. (2016) Redundant Innate and Adaptive Sources of IL17 Production Drive Colon Tumorigenesis. Cancer Res 76:2115-24|
|Sang, Lingjie; Dick, Ivy E; Yue, David T (2016) Protein kinase A modulation of CaV1.4 calcium channels. Nat Commun 7:12239|
|In, Julie; Foulke-Abel, Jennifer; Zachos, Nicholas C et al. (2016) Enterohemorrhagic Escherichia coli reduce mucus and intermicrovillar bridges in human stem cell-derived colonoids. Cell Mol Gastroenterol Hepatol 2:48-62.e3|
|Zachos, Nicholas C; Kovbasnjuk, Olga; Foulke-Abel, Jennifer et al. (2016) Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology. J Biol Chem 291:3759-66|
|Foulke-Abel, Jennifer; In, Julie; Yin, Jianyi et al. (2016) Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology 150:638-649.e8|
|Rais, Rana; Jiang, Weiwei; Zhai, Huihong et al. (2016) FOLH1/GCPII is elevated in IBD patients, and its inhibition ameliorates murine IBD abnormalities. JCI Insight 1:|
|In, Julie G; Foulke-Abel, Jennifer; Estes, Mary K et al. (2016) Human mini-guts: new insights into intestinal physiology and host-pathogen interactions. Nat Rev Gastroenterol Hepatol 13:633-642|
|Hamilton, James P; Koganti, Lahari; Muchenditsi, Abigael et al. (2016) Activation of liver X receptor/retinoid X receptor pathway ameliorates liver disease in Atp7B(-/-) (Wilson disease) mice. Hepatology 63:1828-41|
|Blum, Andrew E; Venkitachalam, Srividya; Guo, Yan et al. (2016) RNA Sequencing Identifies Transcriptionally Viable Gene Fusions in Esophageal Adenocarcinomas. Cancer Res 76:5628-5633|
|Thiele Orberg, E; Fan, H; Tam, A J et al. (2016) The myeloid immune signature of enterotoxigenic Bacteroides fragilis-induced murine colon tumorigenesis. Mucosal Immunol :|
Showing the most recent 10 out of 83 publications