Mouse Physiology Core C is a service core with the purpose of allowing the DDBRTCC Research Base to advance understanding of digestive diseases using mouse models to study physiology and pathophysiology. The Core services are meant to increase the ease and efficiency of studying mouse models, which include transgenic and knock out models including those in which colitis and small intestinal disease occur. In addition, this Core will help our Associate Members who lack experience in using mouse models and have small laboratories, making the labor intensive study of mouse models difficult. The services offered include 1) Advice on establishing and maintaining mouse colonies, including how to breed onto uniform backgrounds;2) Genotyping by PCR, which includes developing and optimizing primers;3) Histological services, which include in vivo paraformaldehyde perfusion for tissue fixation, tissue processing, embedding, sectioning (including cryosectioning), some staining including H&E and PAS, advise on IF. A tissue bank of H &E slides of GI organs ofthe mouse models studied by our Research Base are made available for other Core members to use for preliminary studies;4) Ussing chamber/voltage clamp technology for measuring active ion transport and tight junction permeability and permselectivity is available as is instruction in its use and in calculation ofthe results;5) Metabolic cages are available as is instruction in help in how to use them for metabolic balance studies, including help in blood, urine, stool collections. Three new services are proposed for addition: 1) An experienced mouse pathologist will help review the GI pathology of mouse models, including basal states of transgenic and knock out mice as well as disease models which affect the GI tract;2) A cjrtokine multiplex ELISA assay to analyze cytokine protein levels from human and mice;and 3) a FACS technician to perform analyses for our Research Base members who use similar epithelial and immunologic cells, to increase efficiency and quality control.

Public Health Relevance

The DDBTRCC Mouse Physiology Core is a service Core that has the purpose of helping our Research Base members study mouse models to increase understanding ofthe physiology and pathophysiology of digestive diseases.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
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Special Emphasis Panel (ZDK1)
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Johns Hopkins University
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Bettridge, John; Na, Chan Hyun; Pandey, Akhilesh et al. (2017) H3K4me3 induces allosteric conformational changes in the DNA-binding and catalytic regions of the V(D)J recombinase. Proc Natl Acad Sci U S A 114:1904-1909
Blutt, Sarah E; Broughman, James R; Zou, Winnie et al. (2017) Gastrointestinal microphysiological systems. Exp Biol Med (Maywood) 242:1633-1642
Sunuwar, Laxmi; Asraf, Hila; Donowitz, Mark et al. (2017) The Zn2+-sensing receptor, ZnR/GPR39, upregulates colonocytic Cl- absorption, via basolateral KCC1, and reduces fluid loss. Biochim Biophys Acta 1863:947-960
Jin, Peng; Wang, De-Zhi; Lyu, Chen-Xi et al. (2017) Mismatch Repair Protein hMLH1, but not hMSH2, Enhances Estrogen-Induced Apoptosis of Colon Cancer Cells. J Cancer 8:3232-3241
Anderson, Karen L; Page, Christopher; Swift, Mark F et al. (2017) Nano-scale actin-network characterization of fibroblast cells lacking functional Arp2/3 complex. J Struct Biol 197:312-321
Li, Ling; Piontek, Klaus; Ishida, Masaharu et al. (2017) Extracellular vesicles carry microRNA-195 to intrahepatic cholangiocarcinoma and improve survival in a rat model. Hepatology 65:501-514
Hosoda, Waki; Chianchiano, Peter; Griffin, James F et al. (2017) Genetic analyses of isolated high-grade pancreatic intraepithelial neoplasia (HG-PanIN) reveal paucity of alterations in TP53 and SMAD4. J Pathol 242:16-23
Cha, Boyoung; Yang, Jianbo; Singh, Varsha et al. (2017) PDZ domain-dependent regulation of NHE3 protein by both internal Class II and C-terminal Class I PDZ-binding motifs. J Biol Chem 292:8279-8290
Cil, Onur; Phuan, Puay-Wah; Son, Jung-Ho et al. (2017) Phenylquinoxalinone CFTR activator as potential prosecretory therapy for constipation. Transl Res 182:14-26.e4
Becker, Laren; Nguyen, Linh; Gill, Jaspreet et al. (2017) Age-dependent shift in macrophage polarisation causes inflammation-mediated degeneration of enteric nervous system. Gut :

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