The Center for Synchrotron Biosciences (CSB), with state-of-the-art facilities and laboratories at the National Synchrotron Light Source (NSLS) and at Case Western Reserve University (CWRU), proposes to continue its programs of developing and operating a suite of synchrotron beamlines and associated technologies at Brookhaven National Laboratories (BNL) through a renewal of its P30 Center grant. Since 1994, with continuous funding from the NIH (NCRR/NIBIB), significant support from NSF and in partnership with many academic institutions and BNL, the Center has supported a large, international structural biology user community through the design, construction, and operation of multiple beamlines. The Center serves over 500 life-science users at the NSLS through the continuous upgrade, maintenance, and operation of beamlines that provide photons, advanced end-station instrumentation, and user support and training for a range of synchrotron biophysics technologies at the state-of-the-art. In this renewal proposal, the CSB will continue technology sores for: synchrotron X-ray footprinting, X-ray spectroscopy, and macromolecular crystallography. An Administrative Core will support these cores and Dissemination and Training Cores will supplement their activities. In the Research Base, for the three cores overall, we identify 176 Pls with 245 specific sources of funding (projects) including 230 peer-reviewed project grants (~200 from NIH). We propose to support 145 projects in macromolecular crystallography (100 PIs), 52 projects in X-ray footprinting (43 PIs), and 48 projects in X-ray spectroscopy (33 PIs) for the renewal. The opportunity to considerably enhance the facilities available to this Research Base is based on a once in a generation opportunity provided by the opening of a new synchrotron ring, the NSLS-II. The CSB team, in collaboration with BNL has secured funding for new beamlines at this facility. During the first part of the renewal period, the CSB will complete beamline desig, purchase and fabricate equipment, and then by the mid-point of the grant period complete installation, then commission several state-of-the art beamlines. We will make the transition to vibrant user programs by the end of the grant period with robust scientific facilities, active dissemination and training program and novel scientific results. The outcome of the five-year period will be a Center that continues to be a magnet for world-class synchrotron biosciences research well beyond existing capabilities and productivity that can continue for many years into the future.

Public Health Relevance

The outcome of the five-year period will be a Center that continues to be a magnet for world-class synchrotron biosciences research well beyond existing capabilities and productivity that can continue for many years into the future.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Center Core Grants (P30)
Project #
2P30EB009998-06
Application #
8740761
Study Section
Special Emphasis Panel (ZEB1)
Program Officer
Liu, Christina
Project Start
2009-09-01
Project End
2019-08-31
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Genetics
Type
Schools of Medicine
DUNS #
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Snider, Victoria G; Farquhar, Erik R; Allen, Mark et al. (2017) Design and reactivity of Ni-complexes using pentadentate neutral-polypyridyl ligands: Possible mimics of NiSOD. J Inorg Biochem 175:110-117
Brazzolotto, Deborah; Cantú Reinhard, Fabián G; Smith-Jones, Julian et al. (2017) A High-Valent Non-Heme ?-Oxo Manganese(IV) Dimer Generated from a Thiolate-Bound Manganese(II) Complex and Dioxygen. Angew Chem Int Ed Engl 56:8211-8215
Engelmann, Xenia; Yao, Shenglai; Farquhar, Erik R et al. (2017) A New Domain of Reactivity for High-Valent Dinuclear [M(?-O)2 M'] Complexes in Oxidation Reactions. Angew Chem Int Ed Engl 56:297-301
Sangodkar, Jaya; Perl, Abbey; Tohme, Rita et al. (2017) Activation of tumor suppressor protein PP2A inhibits KRAS-driven tumor growth. J Clin Invest 127:2081-2090
Campbell, Elizabeth A; Kamath, Shreya; Rajashankar, Kanagalaghatta R et al. (2017) Crystal structure of Aquifex aeolicus ?N bound to promoter DNA and the structure of ?N-holoenzyme. Proc Natl Acad Sci U S A 114:E1805-E1814
Carr, Carolyn E; Foster, Andrew W; Maroney, Michael J (2017) An XAS investigation of the nickel site structure in the transcriptional regulator InrS. J Inorg Biochem 177:352-358
Li, Minghui; Zhang, Wei K; Benvin, Nicole M et al. (2017) Structural basis of dual Ca2+/pH regulation of the endolysosomal TRPML1 channel. Nat Struct Mol Biol 24:205-213
Carr, Carolyn E; Musiani, Francesco; Huang, Hsin-Ting et al. (2017) Glutamate Ligation in the Ni(II)- and Co(II)-Responsive Escherichia coli Transcriptional Regulator, RcnR. Inorg Chem 56:6459-6476
Gustavsson, Martin; Wang, Liwen; van Gils, Noortje et al. (2017) Structural basis of ligand interaction with atypical chemokine receptor 3. Nat Commun 8:14135
Massie, Allyssa A; Denler, Melissa C; Cardoso, Luísa Thiara et al. (2017) Equatorial Ligand Perturbations Influence the Reactivity of Manganese(IV)-Oxo Complexes. Angew Chem Int Ed Engl 56:4178-4182

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