The mission of the Cell Imaging and Analysis Facility Core is to provide access to state-of-the-art instrumentation and expertise in confocal microscopy, flow cytometry, cell sorting, and histopathology. Rationale Imaging is an indispensable mode of inquiry for researchers studying the mechanisms by which environmental toxins affect tissues, cells, and subcellular organelles. Recent advances such as fluorescent probes, green fluorescent protein (GFP), confocal optics, two-photon optics, and photoelectronic detectors have improved our ability to image complex tissues. They have enabled us to visualize single molecules and to monitor molecular interactions in live cells. Cost and complexity issues make it impractical for individual investigators to obtain state-of-the-art imaging technologies. Therefore, the need exists for an organized group of specialists with expertise in imaging technologies to acquire the instrumentation, master the applications, and help individual investigators apply the technology to answer specific research questions. Services We support EHSC Investigators to accomplish these tasks: ? Determine the damage resulting from exposure to environmental toxins and other treatments by providing expert pathological analysis from an experienced Veterinary Pathologist. ? Acquire high-resolution images of zebrafish and other models by providing instrumentation, analysis software, infrastructure, and technical assistance. ? Quantify the activation state of signal transduction pathways by developing advanced methodology using flow cytometry, microscopy, and mass spectrometry. ? Isolate specific cell populations from cell culture, blood, and tissue by providing instrumentation and technical assistance.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES000210-45
Application #
8448285
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
45
Fiscal Year
2013
Total Cost
$115,842
Indirect Cost
$36,606
Name
Oregon State University
Department
Type
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339
Cooper, T K; Murray, K N; Spagnoli, S et al. (2015) Primary intestinal and vertebral chordomas in laboratory zebrafish (Danio rerio). Vet Pathol 52:388-92
Vasil'ev, Yury V; Tzeng, Shin-Chen; Huang, Lin et al. (2014) Protein modifications by electrophilic lipoxidation products: adduct formation, chemical strategies and tandem mass spectrometry for their detection and identification. Mass Spectrom Rev 33:157-82
Siddens, Lisbeth K; Krueger, Sharon K; Henderson, Marilyn C et al. (2014) Mammalian flavin-containing monooxygenase (FMO) as a source of hydrogen peroxide. Biochem Pharmacol 89:141-7
Barsamian, Adam L; Perkins, Matt J; Field, Jennifer A et al. (2014) Regioselective syntheses of [13C]4-labelled sodium 1-carboxy-2-(2-ethylhexyloxycarbonyl)ethanesulfonate and sodium 2-carboxy-1-(2-ethylhexyloxycarbonyl)ethanesulfonate from [13C]4-maleic anhydride. J Labelled Comp Radiopharm 57:397-401
Zhang, Jie; He, Yunteng; Freund, William M et al. (2014) Electron Diffraction of Superfluid Helium Droplets. J Phys Chem Lett 5:1801-1805
Miller, Galen W; Truong, Lisa; Barton, Carrie L et al. (2014) The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome. Comp Biochem Physiol Part D Genomics Proteomics 10:22-9
Gouthu, Satyanarayana; O'Neil, Shawn T; Di, Yanming et al. (2014) A comparative study of ripening among berries of the grape cluster reveals an altered transcriptional programme and enhanced ripening rate in delayed berries. J Exp Bot 65:5889-902
Anderson, Kim A; Seck, Dogo; Hobbie, Kevin A et al. (2014) Passive sampling devices enable capacity building and characterization of bioavailable pesticide along the Niger, Senegal and Bani Rivers of Africa. Philos Trans R Soc Lond B Biol Sci 369:20130110
Lebold, Katie M; Traber, Maret G (2014) Interactions between *-tocopherol, polyunsaturated fatty acids, and lipoxygenases during embryogenesis. Free Radic Biol Med 66:13-9
Padmanarayana, Murugesh; Hams, Nicole; Speight, Lee C et al. (2014) Characterization of the lipid binding properties of Otoferlin reveals specific interactions between PI(4,5)P2 and the C2C and C2F domains. Biochemistry 53:5023-33

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