The overall objective of the Molecular Cell and Analytical Services Facility Core (MCASFC) is to provide Center members with education on, and access to, state-of-the-art tools for assessing the effects of the environment on human and mammalian genomes and epigenomes. The Core also provides analytical services, particularly those for metals analysis in cells and tissues of exposed organisms, since metal effects on the genome and epigenome are specialized research strengths of the Center. The rationale for, and continuing evolution of, this Core reflects current trends in environmental health research, and in particular the rapidly changing field of gene x environment interactions. Functionally important, dose-related perturbations to human health from environmental stressors (e.g. metals, carcinogens, air pollution particulates) are being actively analyzed by Center researchers who study changes in gene expression, epigenetic regulation at candidate gene and whole genome levels, cell cycle perturbations, protein expression and signaling pathway interactions. The MCASFC supports Center investigators' funded studies using genomics and epigenomics to identify biomarkers and predictive sentinels of toxic challenges to health, as well as to identify targets for preventive and therapeutic interventions. One new initiative implemented in 2008 and overseen by this core is the collaborative yeast screen project involving analysis of global gene expression changes induced by metals and ozone. This new project, supported in part by the Center Director's fund, involved multiple Center investigators with Dr. Thomas Begley at the GeNYsis Center in Albany, NY. It has become increasingly evident that the epigenome plays an enormous role in modulating the responses of organisms to environmental exposures. Thus, other new initiatives that have developed for this Core since the last renewal are the incorporation of ChlP-Chip, ChlP-Seq and RNA-Seq for epigenetic and transcriptome biomarker discovery and for investigating mechanisms underlying toxic and disease responses to environmental agents. Research on genome/epigenome x environment interactions requires multidisciplinary approaches and utilization of highly sensitive molecular techniques supported by state-of-the-art instruments and emerging technologies. The MCASFC provides Center investigators with evolving access to specialized methodologies and equipment, either on-site or off-site, by taking advantage of regional resources involving intra- and interuniversity research partnerships. The MCASFC acquires new equipment as deemed necessary, by continuing to leverage funds from competitive and institutional funding sources. Furthermore, since genome/epigenome studies require considerable training prior to implementation, the Facility Core provides Center investigators with consultation services on the uses, applications, limitations and challenges ofthe latest techniques.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES000260-51
Application #
8831656
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
2016-03-31
Budget Start
2015-04-01
Budget End
2016-03-31
Support Year
51
Fiscal Year
2015
Total Cost
$149,766
Indirect Cost
$56,162
Name
New York University
Department
Type
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Lim, Chris C; Thurston, George D; Shamy, Magdy et al. (2018) Temporal variations of fine and coarse particulate matter sources in Jeddah, Saudi Arabia. J Air Waste Manag Assoc 68:123-138
Lee, Hyun-Wook; Park, Sung-Hyun; Weng, Mao-Wen et al. (2018) E-cigarette smoke damages DNA and reduces repair activity in mouse lung, heart, and bladder as well as in human lung and bladder cells. Proc Natl Acad Sci U S A 115:E1560-E1569
Muñoz, Alexandra; Eldridge, Will J; Jakobsen, Nina Munkholt et al. (2018) Cellular shear stiffness reflects progression of arsenic-induced transformation during G1. Carcinogenesis 39:109-117
Harley, Naomi H (2018) Effect of Residential Radon Decay Product Dose Factor Variability on Reporting of Dose. Health Phys 114:398-407
Roy, Nirmal K; Candelmo, Allison; DellaTorre, Melissa et al. (2018) Characterization of AHR2 and CYP1A expression in Atlantic sturgeon and shortnose sturgeon treated with coplanar PCBs and TCDD. Aquat Toxicol 197:19-31
Klocke, Carolyn; Allen, Joshua L; Sobolewski, Marissa et al. (2018) Exposure to fine and ultrafine particulate matter during gestation alters postnatal oligodendrocyte maturation, proliferation capacity, and myelination. Neurotoxicology 65:196-206
Farzan, Shohreh F; Howe, Caitlin G; Chen, Yu et al. (2018) Prenatal lead exposure and elevated blood pressure in children. Environ Int 121:1289-1296
Roy, Nirmal K; DellaTorre, Melissa; Candelmo, Allison et al. (2018) Characterization of AHR1 and its functional activity in Atlantic sturgeon and shortnose sturgeon. Aquat Toxicol 205:25-35
Lim, Chris C; Hayes, Richard B; Ahn, Jiyoung et al. (2018) Association between long-term exposure to ambient air pollution and diabetes mortality in the US. Environ Res 165:330-336
Fan, Xiaozhou; Alekseyenko, Alexander V; Wu, Jing et al. (2018) Human oral microbiome and prospective risk for pancreatic cancer: a population-based nested case-control study. Gut 67:120-127

Showing the most recent 10 out of 407 publications