7C. GENOMICS AND IMAGING FACILITIES CORE INTRODUCTION AND SPECIFIC AIMS The CEHS Genomics and Bioinformatics Facilities Core was created in September 2001 to provide CEHS members with integrated facilities for high-throughput, data-intensive genomics as well as bioinformatics analysis, large-scale database storage and management, data mining and data modeling required to fully implement systems approaches to studying the biological impact of environmental factors. In response to the evolution of Center member research needs, a new CEHS Imaging Facility was integrated with the CEHS Genomics and Bioinformatics Facilities Core last year, and this CEHS facilities core has been renamed the """"""""Genomics and Imaging Facilities Core."""""""" The Core now provides CEHS members with the tools and expertise for high-resolution and high-throughput imaging integrated with data-intensive genomics and bioinformatics. This powerful combination is coupled with flexible and accessible data sharing and a strong commitment to new technology development and deployment to keep the CEHS at the cutting edge of high-throughput imaging and analytical methods. The Genomics and Imaging Facilities Core operates as both a training resource and as a service and developmental laboratory, with the following Specific Aims: (1) To provide integrated support for genomics technologies including massively parallel sequencing and oligonucleotide arrays. (2) To provide customized imaging options and the resources for high-resolution images and high-throughput screening using imaging capabilities. (3) To provide integrative support for high-throughput screening of factors with environmental impact on cellular function, including support for flexible automated fluidics, highly parallel real-time PCR, fluorescence, luminescence, absorbance, and single-cell imaging and quantification. (4) To support the bioinformatic frameworks necessary to interpret and manage these large data sets. (5) To provide and maintain network servers and storage devices to facilitate the transfer and analysis of information-rich data sets. (6) To provide training with new genomic and imaging technologies as well as commercial and open-source bioinformatics software. (7) To work with Center members to develop and acquire new technologies and to apply their results to develop data models and quality control procedures and error modeling.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES002109-33
Application #
8466330
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
33
Fiscal Year
2013
Total Cost
$231,197
Indirect Cost
$91,126
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Moore, Christopher L; Papa 3rd, Louis J; Shoulders, Matthew D (2018) A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo. J Am Chem Soc 140:11560-11564
Wang, Xin; Garcia, Carlos T; Gong, Guanyu et al. (2018) Automated Online Solid-Phase Derivatization for Sensitive Quantification of Endogenous S-Nitrosoglutathione and Rapid Capture of Other Low-Molecular-Mass S-Nitrosothiols. Anal Chem 90:1967-1975
Tam, Brooke E; Hao, Yining; Sikes, Hadley D (2018) An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles. Biotechnol Prog 34:1589-1595
Chan, Cheryl; Pham, Phuong; Dedon, Peter C et al. (2018) Lifestyle modifications: coordinating the tRNA epitranscriptome with codon bias to adapt translation during stress responses. Genome Biol 19:228
Li, Weiwei; Chan, Chi-Kong; Liu, Yushuo et al. (2018) Aristolochic Acids as Persistent Soil Pollutants: Determination of Risk for Human Exposure and Nephropathy from Plant Uptake. J Agric Food Chem 66:11468-11476
Ge, Zhongming; Sheh, Alexander; Feng, Yan et al. (2018) Helicobacter pylori-infected C57BL/6 mice with different gastrointestinal microbiota have contrasting gastric pathology, microbial and host immune responses. Sci Rep 8:8014
Yuan, Yifeng; Hutinet, Geoffrey; Valera, Jacqueline Gamboa et al. (2018) Identification of the minimal bacterial 2'-deoxy-7-amido-7-deazaguanine synthesis machinery. Mol Microbiol 110:469-483
Mannion, Anthony; Shen, Zeli; Fox, James G (2018) Comparative genomics analysis to differentiate metabolic and virulence gene potential in gastric versus enterohepatic Helicobacter species. BMC Genomics 19:830
Kulik, Heather J (2018) Large-scale QM/MM free energy simulations of enzyme catalysis reveal the influence of charge transfer. Phys Chem Chem Phys 20:20650-20660
Rizzo, Alessandro A; Vassel, Faye-Marie; Chatterjee, Nimrat et al. (2018) Rev7 dimerization is important for assembly and function of the Rev1/Pol? translesion synthesis complex. Proc Natl Acad Sci U S A 115:E8191-E8200

Showing the most recent 10 out of 970 publications