The goals of the Morphology &Microscopic Imaging Module are to make available to vision researchers the instruments and expertise required (1) to perform microscopic analyses of ocular specimens, subcellular fractions, or cultured cells, and (2) to generate images of microscopic preparations for analysis, publication, presentation and display. The heart of the Module is a centralized facility for basic microscopy where tissues are processed and blocks are sectioned. This is essential for our group since virtually all our lab scientists must examine cell or tissue structure for some aspects of their work, but individual investigators cannot justify or support a fully-equipped microscopy lab -- or the ongoing salaries of skilled microscopy technicians. In addition to supporting tissue processing, a key service of the Module is to facilitate access to institutional centers for electron microscopy and confocal imaging. This latter activity is in keeping with the overall strategy for our Core program emphasized in this budget cycle: to use our Modules to help investigators take advantage of institutional research resources thereby enhancing productivity by providing access to costly instruments that could not be individually supported while integrating our Core vision group with the broader research community on campus.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY001931-36
Application #
8433886
Study Section
Special Emphasis Panel (ZEY1-VSN (10))
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
36
Fiscal Year
2012
Total Cost
$98,896
Indirect Cost
$34,258
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Lapierre-Landry, Maryse; Huckenpahler, Alison L; Link, Brian A et al. (2018) Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography. Transl Vis Sci Technol 7:4
Patterson, Emily J; Kalitzeos, Angelos; Kasilian, Melissa et al. (2018) Residual Cone Structure in Patients With X-Linked Cone Opsin Mutations. Invest Ophthalmol Vis Sci 59:4238-4248
Davidson, Benjamin; Kalitzeos, Angelos; Carroll, Joseph et al. (2018) Automatic Cone Photoreceptor Localisation in Healthy and Stargardt Afflicted Retinas Using Deep Learning. Sci Rep 8:7911
Reid, Christopher A; Nettesheim, Emily R; Connor, Thomas B et al. (2018) Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMD. Sci Rep 8:11763
Zhang, Hanmeng; Mu, Lianwei; Wang, Dandan et al. (2018) Uncovering a critical period of synaptic imbalance during postnatal development of the rat visual cortex: role of brain-derived neurotrophic factor. J Physiol 596:4511-4536
Lewis, Tylor R; Kundinger, Sean R; Link, Brian A et al. (2018) Kif17 phosphorylation regulates photoreceptor outer segment turnover. BMC Cell Biol 19:25
Huckenpahler, Alison; Wilk, Melissa; Link, Brian et al. (2018) Repeatability and Reproducibility of In Vivo Cone Density Measurements in the Adult Zebrafish Retina. Adv Exp Med Biol 1074:151-156
Lewis, Tylor R; Zareba, Mariusz; Link, Brian A et al. (2018) Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1. Mol Biol Cell 29:180-190
Lee, Daniel J; Woertz, Erica N; Visotcky, Alexis et al. (2018) The Henle Fiber Layer in Albinism: Comparison to Normal and Relationship to Outer Nuclear Layer Thickness and Foveal Cone Density. Invest Ophthalmol Vis Sci 59:5336-5348
Linderman, Rachel E; Muthiah, Manickam N; Omoba, Sarah B et al. (2018) Variability of Foveal Avascular Zone Metrics Derived From Optical Coherence Tomography Angiography Images. Transl Vis Sci Technol 7:20

Showing the most recent 10 out of 517 publications