This application requests renewal of a Core Grant to support 25 vision research investigators with 15 qualifying NEI R01 grants, 2 NEI R21 grants, 1 U10 grant, 1 NEI-funded Research Development Center grant (PN1) and 2 NEI-funded training (T32) grants at Baylor College of Medicine. In this competing renewal application, we report our highly successful and productive past usage of the Core facilities (with a large number of publications benefitting from the Core Modules and high level of collaborations among Core members), and seek continued support for three modules based on the current needs and emerging demand for cutting-edge technology of the vision research community. The proposed Modules are Microscopy, Digital Imaging &Histology;Machine Shop;and Small Animal Visual System Analysis.
The aim of the proposal is to provide support and facilities, essential for progress on NEI-funded projects, net available among the departments and which cannot be obtained through individual research grants.
The specific aims of the application are (1) Te provide a state-of-the-art scanning confocal microscope for high resolution three-dimensional imaging and an advanced transmission electron microscope for ultrastructural analysis of ocular and neural tissues, and molecular imaging. (2) To provide facilities and services to process digital images obtained from confocal, conventional light and electron microscopes;histological facilities, and services and training for both light and electron microscopy. (3) To provide in-house machine shop services to the Vision Core investigators who need special apparatus not commercially available. (4) To design and fabricate high-precision devices for vision Core laboratories with the new state-of-the-art 5-axes machine. (5) To provide facilities not available in individual laboratories for characterizing animal models (mice rats and rabbits) of ocular disease. These include instruments for biochemical analysis of proteins and small molecules, and functional analysis of vision using electroretinography (ERG) and opto-metor reflex assays (6) To foster collaborations among vision researcher groups at Baylor through sharing of common facilities. (7) To provide a supportive environment to attract and retain outstanding vision research faculty, students and postdoctoral fellows;and facilitate new vision research projects.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY002520-34
Application #
8502662
Study Section
Special Emphasis Panel (ZEY1-VSN (03))
Program Officer
Liberman, Ellen S
Project Start
1997-07-01
Project End
2015-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
34
Fiscal Year
2013
Total Cost
$559,799
Indirect Cost
$195,109
Name
Baylor College of Medicine
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Reimer, Jacob; Froudarakis, Emmanouil; Cadwell, Cathryn R et al. (2014) Pupil fluctuations track fast switching of cortical states during quiet wakefulness. Neuron 84:355-62
Deng, Ruzhi; Su, Zhitao; Lu, Fan et al. (2014) A potential link between bacterial pathogens and allergic conjunctivitis by dendritic cells. Exp Eye Res 120:118-26
Bui, Diem K; Carvounis, Petros E (2014) Evidence for and against intravitreous corticosteroids in addition to intravitreous antibiotics for acute endophthalmitis. Int Ophthalmol Clin 54:215-24
Karandikar, Umesh C; Jin, Meng; Jusiak, Barbara et al. (2014) Drosophila eyes absent is required for normal cone and pigment cell development. PLoS One 9:e102143
Agosto, Melina A; Zhang, Zhixian; He, Feng et al. (2014) Oligomeric state of purified transient receptor potential melastatin-1 (TRPM1), a protein essential for dim light vision. J Biol Chem 289:27019-33
Zhang, Xiaobo; De Paiva, Cintia S; Su, Zhitao et al. (2014) Topical interferon-gamma neutralization prevents conjunctival goblet cell loss in experimental murine dry eye. Exp Eye Res 118:117-24
McClellan, Andrew J; Volpe, Eugene A; Zhang, Xiaobo et al. (2014) Ocular surface disease and dacryoadenitis in aging C57BL/6 mice. Am J Pathol 184:631-43
Froudarakis, Emmanouil; Berens, Philipp; Ecker, Alexander S et al. (2014) Population code in mouse V1 facilitates readout of natural scenes through increased sparseness. Nat Neurosci 17:851-7
Ecker, Alexander S; Berens, Philipp; Cotton, R James et al. (2014) State dependence of noise correlations in macaque primary visual cortex. Neuron 82:235-48
El Annan, Jaafar; Carvounis, Petros E (2014) Current management of vitreous hemorrhage due to proliferative diabetic retinopathy. Int Ophthalmol Clin 54:141-53

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