The objective of this application is to provide established NEI-funded researchers with additional, shared support to enhance their individual research capabilities. A further goal is to enhance the research capability of Washington University by fostering collaborative studies and attracting scientists new to vision research. These objectives will be achieved by operating four research/service cores, which will provide the following services. 1. A Morphology and Imaging Core will provide technical support in the preparation of ocular tissues for analysis by light or electron microscopy. The core also provides in situ hybridization, immunocytochemistry and laser micro-dissection. Core investigators have unlimited access to confocal and multiphoton microscopes. For in vivo imaging/measurements, a fluorotron, fluorescence macroscope, and small animal Optical Coherence Tomography (OCT) system are available. 2. A Visual Function Testing Core will provide expertise, instrumentation, and training on technologies used to quantify visual performance in mice and other model systems. ERG, VEP and optometry analysis are supported. This core also includes technical support for the fabrication, diagnosis and repair of electronic equipment. 3. A Biostatistics Core will provide statistical and methodological expertise in study design and assure the validity of statistical analyses and reported results. This core will also assist in the training of residents and clinicians in areas of clinical research methodology. 4. A Molecular Genetics Core will provide customized services for the production of transgenic and knockout mice using Crispr/Cas9 technology. Supported services also include assistance with design and preparation of constructs for gene targeting, preparation of DNA clones and probes, design of polymerase chain reaction components, microinjection services (IVF), and sperm/embryo cryopreservation. Provision of these support services and resources will greatly enhance the research capabilities of investigators at Washington University and will facilitate collaboration among new and established vision scientists.

Public Health Relevance

Washington University supports a large and diverse group of vision scientists who, collectively, make a substantial contribution to the diagnosis and treatment of blinding eye disease. The provision of shared resources and technical support made possible through the Center Core allows investigators to make far more rapid scientific progress than otherwise be possible.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Washington University
Saint Louis
United States
Zip Code
Ban, Norimitsu; Siegfried, Carla J; Apte, Rajendra S (2018) Monitoring Neurodegeneration in Glaucoma: Therapeutic Implications. Trends Mol Med 24:7-17
Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration. J Lipid Res 59:1414-1423
Buckingham, Erin M; Foley, Maria A; Grose, Charles et al. (2018) Identification of Herpes Zoster-Associated Temporal Arteritis Among Cases of Giant Cell Arteritis. Am J Ophthalmol 187:51-60
Stunkel, Leanne; Kung, Nathan H; Wilson, Bradley et al. (2018) Incidence and Causes of Overdiagnosis of Optic Neuritis. JAMA Ophthalmol 136:76-81
Gordon, Mae O; Kass, Michael A (2018) What We Have Learned From the Ocular Hypertension Treatment Study. Am J Ophthalmol 189:xxiv-xxvii
Andley, Usha P; Tycksen, Eric; McGlasson-Naumann, Brittney N et al. (2018) Probing the changes in gene expression due to ?-crystallin mutations in mouse models of hereditary human cataract. PLoS One 13:e0190817
Vinberg, Frans; Peshenko, Igor V; Chen, Jeannie et al. (2018) Guanylate cyclase-activating protein 2 contributes to phototransduction and light adaptation in mouse cone photoreceptors. J Biol Chem 293:7457-7465
Gordon, Mae O; Higginbotham, Eve J; Heuer, Dale K et al. (2018) Assessment of the Impact of an Endpoint Committee in the Ocular Hypertension Treatment Study. Am J Ophthalmol :
Ruzycki, Philip A; Zhang, Xiaodong; Chen, Shiming (2018) CRX directs photoreceptor differentiation by accelerating chromatin remodeling at specific target sites. Epigenetics Chromatin 11:42
Brightman, Diana S; Grant, Rachel L; Ruzycki, Philip A et al. (2018) MLL1 is essential for retinal neurogenesis and horizontal inner neuron integrity. Sci Rep 8:11902

Showing the most recent 10 out of 696 publications