Non-invasive assessment of eye structure and function is essential to both basic and translational research in vision science. Widely used technologies include slit lamp for biomicroscopy of anterior and posterior segment, electroretinography (ERG, for massed retinal signal separable into components), optical coherence tomography (OCT) for display of layered tissues in posterior and anterior segments, imaging of the fundus via multiple modes of visualization (color, autofluorescence, dye-based angiography, infrared reflectance), and optokinetic nystagmus (to assess visuomotor control, visual acuity and contrast sensitivity). In response to growing UAB vision researcher needs, a new ?Ocular Phenotyping Core? was established to encompass a comprehensive suite of instrumentation and to provide the necessary support for a qualified PhD staff member to assist in accurate ocular phenotyping. Specific instruments include Bioptigen 840 nm SD-OCT and Micron IV digital fundus camera for small animals, Spectralis SDOCT for large animals and human donor eyes, and Optomotry optokinetic nystagmus in small animals including mice and zebrafish. This core will support 15 UAB Vision Scientists, including 13 with planned moderate to extensive use and 9 who are currently NEI R01-funded. Review of publications during the two years prior to this application reveals excellent productivity using these collaboratively purchased instruments, justifying the formalizing of these activities as a core. The Director and Associate Director of this proposed core have extensive publication experience in electroretinography and OCT validation/ interpretation, respectively. New directions for the core include the establishment of MRI imaging for calcium flux in rodent eyes. Additionally an ?Ocular Phenotyping Fair? will be implemented to identify new ocular mouse models through full ocular phenotyping screens of mouse models generated by non-ocular scientists that were originally generated to answer questions pertinent to thier organ systems of interest.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY003039-36A1
Application #
9153116
Study Section
Special Emphasis Panel (ZEY1-VSN (03))
Project Start
Project End
Budget Start
2016-08-01
Budget End
2017-07-31
Support Year
36
Fiscal Year
2016
Total Cost
$43,191
Indirect Cost
$13,809
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
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Litts, Katie M; Zhang, Yuhua; Freund, K Bailey et al. (2018) OPTICAL COHERENCE TOMOGRAPHY AND HISTOLOGY OF AGE-RELATED MACULAR DEGENERATION SUPPORT MITOCHONDRIA AS REFLECTIVITY SOURCES. Retina 38:445-461
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Gawne, Timothy J; Ward, Alexander H; Norton, Thomas T (2018) Juvenile Tree Shrews Do Not Maintain Emmetropia in Narrow-band Blue Light. Optom Vis Sci 95:911-920
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Gu, Boyu; Wang, Xiaolin; Twa, Michael D et al. (2018) Noninvasive in vivo characterization of erythrocyte motion in human retinal capillaries using high-speed adaptive optics near-confocal imaging. Biomed Opt Express 9:3653-3677
Panthi, Shyam; Chen, Jianzhong; Wilson, Landon et al. (2018) Detection of Lipid Mediators of Inflammation in the Human Tear Film. Eye Contact Lens :
Strang, Christianne E; Ray, Mary Katherine; Boggiano, Mary M et al. (2018) Effects of tDCS-like electrical stimulation on retinal ganglion cells. Eye Brain 10:65-78
Markert, John E; Jasien, Jessica V; Turner, Daniel C et al. (2018) IOP, IOP Transient Impulse, Ocular Perfusion Pressure, and Mean Arterial Pressure Relationships in Nonhuman Primates Instrumented With Telemetry. Invest Ophthalmol Vis Sci 59:4496-4505

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